Optimization of extraction process of chitin from Periostracum Cicadae and evaluation of antioxidant activity and hemostatic effect
In this study,the extraction process of chitin from Periostracum Cicadae was optimized by single factor and orthogo-nal experiment.The antioxidant activity of the extracts was evaluated with ABTS、DPPH and O2 free radical clearance.The he-mostatic effect of the extracts was evaluated by the effect of the extract on rabbit blood coagulation time,bleeding time and bleeding weight of different bleeding models.The results showed that the optimal extraction process of chitin was as follows:decalcification:mass fraction:8％HCl,temperature:room temperature,solid-liquid ratio:1∶25,ultrasonic power:160 W,ultra-sonic time:150 min;deproteinization:mass fraction:10％NaOH,temperature:60 ℃,solid-liquid ratio:1∶25,ultrasonic pow-er:no significant difference,ultrasonic time:90 min;decolorization:mass fraction:5％KMnO4,8％NaHSO3,temperature:90 ℃,time:KMnO4 reaction 60 min,NaHSO3 reaction 90 min;The chitin can clear ABTS,DPPH and O2 free radical.Compared with Yunnan Baiyao powder at the same dose,chitin has better in vitro coagulant promoting ability,chitin can significantly shorten the bleeding time and amount of bleeding of rat tail and the bleeding time of liver wound.This study can provide refer-ence for the high-value utilization and comprehensive development of insect traditional Chinese medicine.
万方数据
维普
