To establish a UPLC fingerprint of the extract of Drynariae Rhizoma before and after processing,and to study the spectrum-effect relationship between the extract of Drynariae Rhizoma and alkaline phosphatase(ALP)activity in osteosarco-ma MG63 cell line.Using UPLC method to establish the fingerprint of raw materials and processing slices of Drynariae Rhizo-ma and identified their chemical components by UPLC-Q-TOF MS;The ALP activity was measured to determine the effect of Drynariae Rhizoma extract on osteosarcoma MG63 cell line;Grey correlation analysis,bivariate correlation analysis,and partial least square regression analysis were used to analyze the spectrum-effect relationship between the UPLC characteristic finger-print peaks and ALP activity of the raw and processed products of Drynariae Rhizoma.Among the 12 characteristic fingerprint peaks calibrated,it was determined that peaks 4,5 and 12 were positively correlated with ALP activity,and the corresponding substances were caffeic acid 4-O-β-D-glucopyranoside,catechin 7-glucoside and naringin.It provides a reference for in-depth research on the active material basis of Drynariae Rhizoma in promoting osteogenic differentiation effect.
关键词
骨碎补/UPLC指纹图谱/碱性磷酸酶活性/谱效关系/人骨肉瘤细胞MG63
Key words
Drynariae Rhizoma/UPLC fingerprint/alkaline phosphatase activity/spectrum-effect relationship/osteosarcoma MG63 cell line
维普
万方数据