橄榄苦苷酶水解产物具有良好的生物活性,为了促进纤维素酶水解橄榄苦苷,本研究通过天然低共熔溶剂(natural deep eutectic solvent,NADES)对纤维素酶稳定性的促进作用,形成NADES-纤维素酶溶剂系统促进橄榄苦苷的酶水解.以橄榄苦苷酶水解率为指标,考察温度、pH和DES种类及浓度对纤维素酶水解橄榄苦苷的影响.结果表明:在50 ℃,pH=5时水解3 h,以10％(V/V)DES-5(甜菜碱:1,4-丁二醇=1∶2,n/n)为溶剂系统的橄榄苦苷酶水解率是缓冲液的1.7倍;证明以氯化胆碱或甜菜碱为氢键受体(hydrogen bond acceptor,HBA),多元醇为氢键供体(hydrogen bond donor,HBD)的NADES对增强纤维素酶稳定性、促进橄榄苦苷酶水解具有良好的效果,为高效制备橄榄苦苷酶水解产物提供了科学依据.
Study on the promoting effect of natural deep eutectic solvent on cellulase hydrolysis of oleuropein
Oleuropein hydrolyzed products exhibit potent biological activities.To enhance oleuropein hydrolysis by cellulase,a natural deep eutectic solvent(NADES)was utilized to stabilize the cellulase enzyme.The DES-cellulase solvent system effec-tively promoted enzymatic hydrolysis of oleuropein.Factors such as temperature,pH,DES type and concentration were studied to determine their impact on the hydrolysis rate.Results showed that at 50 ℃ and pH=5,the hydrolysis rate of oleuropein was 1.7 times higher than that of buffer solution when using 10％(V/V)DES-5(betaine:1,4-butanediol=1∶2,n/n)as solvent for 3 h.This study confirms that NADESs with choline chloride or betaine as hydrogen bond acceptor(HBA)and polyols as hydrogen bond donor(HBD)effectively enhance cellulase stability and promote oleuropein hydrolysis.These find-ings provide a scientific basis for the efficiently producing oleuropein enzyme hydrolyzed products.
deep eutectic solventoleuropeincellulaseenzymatic hydrolysis
万方数据
维普
