摘要
橄榄苦苷酶水解产物具有良好的生物活性,为了促进纤维素酶水解橄榄苦苷,本研究通过天然低共熔溶剂(natural deep eutectic solvent,NADES)对纤维素酶稳定性的促进作用,形成NADES-纤维素酶溶剂系统促进橄榄苦苷的酶水解.以橄榄苦苷酶水解率为指标,考察温度、pH和DES种类及浓度对纤维素酶水解橄榄苦苷的影响.结果表明:在50 ℃,pH=5时水解3 h,以10％(V/V)DES-5(甜菜碱:1,4-丁二醇=1∶2,n/n)为溶剂系统的橄榄苦苷酶水解率是缓冲液的1.7倍;证明以氯化胆碱或甜菜碱为氢键受体(hydrogen bond acceptor,HBA),多元醇为氢键供体(hydrogen bond donor,HBD)的NADES对增强纤维素酶稳定性、促进橄榄苦苷酶水解具有良好的效果,为高效制备橄榄苦苷酶水解产物提供了科学依据.
Abstract
Oleuropein hydrolyzed products exhibit potent biological activities.To enhance oleuropein hydrolysis by cellulase,a natural deep eutectic solvent(NADES)was utilized to stabilize the cellulase enzyme.The DES-cellulase solvent system effec-tively promoted enzymatic hydrolysis of oleuropein.Factors such as temperature,pH,DES type and concentration were studied to determine their impact on the hydrolysis rate.Results showed that at 50 ℃ and pH=5,the hydrolysis rate of oleuropein was 1.7 times higher than that of buffer solution when using 10％(V/V)DES-5(betaine:1,4-butanediol=1∶2,n/n)as solvent for 3 h.This study confirms that NADESs with choline chloride or betaine as hydrogen bond acceptor(HBA)and polyols as hydrogen bond donor(HBD)effectively enhance cellulase stability and promote oleuropein hydrolysis.These find-ings provide a scientific basis for the efficiently producing oleuropein enzyme hydrolyzed products.
基金项目
甘肃省科技重大专项(22ZD6FA021)
兰州市科技计划(2022-2-2)
甘肃省重点研发计划(22YF7NA027)
云南省重点研发计划(202203AD150012)
维普
万方数据