首页|不同产地肉桂质量及炮制过程成分变化研究

不同产地肉桂质量及炮制过程成分变化研究

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建立了肉桂指纹图谱和5种有效成分的含量测定方法,对不同产地28批肉桂药材差异及炮制前后的成分变化进行了研究.采用超高效液相色谱法建立了肉桂指纹图谱并对共有峰进行了指认,采用OriginPro2023软件对28批样品指纹图谱进行了聚类分析和主成分分析,聚类分析的样本和共有峰分类情况与主成分分析情况相近,其中阳春市、肇庆市高要区、防城港市和贵港市样本分类较为集中,采用SIMCA14.1软件进行了正交偏最小二乘判别分析,可实现对广东、广西两省/自治区肉桂的有效区分.通过香豆素、肉桂醇、肉桂酸、桂皮醛、2-甲氧基桂皮醛及挥发油的含量测定对炮制前后的肉桂样本进行对比分析,结果表明,药材与饮片5个成分和挥发油含量均值较为相近,肉桂炮制过程的成分差异较小;且各批次间桂皮醛、肉桂酸和肉桂醇的整体变化趋势相近,2-甲氧基桂皮醛和香豆素的变化趋势相近,与化学计量学分析结果一致.本研究可为肉桂产地选择、质量研究提供参考.
Study on the quality of Cinnamomi Cortex from different origins and the changes of components in the processing process
The fingerprint of Cinnamomi Cortex was established by ultra high performance liquid chromatography(UPLC)and the common peaks were identified.The cluster analysis(CA)and principal component analysis(PC A)of 28 batches of sam-ples were carried out by OriginPro 2023 software.The classification of samples and common peaks of CA is similar to that of PCA,among which the sample classification of Yangchun City,Gaoyao District of Zhaoqing City,Fangchenggang City and Guigang City is more concentrated.Orthogonal partial least squares-discriminant analysis(OPLS-DA)was carried out by u-sing SIMCA14.1 software,which can effectively distinguish Cinnamomi Cortex from Guangdong and Guangxi provinces.The samples of Cinnamomi Cortex before and after processing were compared and analyzed by determining the content of couma-rin,cinnamyl alcohol,cinnamic acid,cinnamaldehyde,2-methoxycinnamaldehyde and volatile oil.The results showed that the average content of five components and volatile oil between medicinal materials and decoction pieces were similar,and there was little difference in the composition of Cinnamomi Cortex in the processing process.The overall change trends of cinnamal-dehyde,cinnamic acid and cinnamyl alcohol were similar,and the changes of 2-methoxycinnamaldehyde and coumarin were similar,which were consistent with the results of chemometrics analysis.This study can provide reference for the selection of origins and quality research of Cinnamomi Cortex.

Cinnamomi Cortexprocessingcinnamaldehydechemometricscontent determination

梅菊、吴涛、李强、刘洋、殷涛、廖香莲、孙代华、胡力飞

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湖北省中药配方颗粒工程技术研究中心,黄石 435100

湖北师范大学生命科学学院,黄石 435002

湖北工业大学教育部工业发酵省部共建协同创新中心,武汉 430068

肉桂 炮制 桂皮醛 化学计量学 含量测定

湖北省技术创新专项湖北省科技重大专项

2019AEE0012020ACA007-03

2024

天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCD北大核心
影响因子:0.783
ISSN:1001-6880
年,卷(期):2024.36(3)
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