To study the chemical constituents of the herbaceous stems of Ephedra intermedia Schrenk et C.A.Mey.Twenty-two compounds were isolated from the 50％aqueous acetone extract of Ephedra intermedia by silica gel,sephadex LH-20 column chromatography,Toyopreal HW-40C column chromatography,as well as semi-preparative HPLC methods.Their structures were identified by spectral analysis as 4-methoxycinnamic acid(1),cinnamic acid(2),ω-hydroxypropioguaiacone(3),threo-8S-7-methoxysyringylglycerol(4),3-(2,4'-dihydroxy-3',5'-dimethoxyphenyl)propanoic acid(5),8-hydroxy-9-methyl-7-(4-hydroxy-3,5-dimethoxyphenyl)-7-propanone(6),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(7),2-hydroxyl-1-[4-hydroxy-3-methoxyphenyl(propan-1-one)](8),methyl caffeate(9),trans-syringin(10),cis-syringin(11),(4R)-4-hydroxy-4-(2-hydroxypropan-2-yl)cyclo-hex-1-ene-1-carboxylic acid(12),7-hydroxyterpineol(13),dehydrovomi-foliol(14),abscisic acid(15),loliolide(16),(E)-3-(3-hydroxybut-l-enyl)-2,4,4-trimethylcyclohexa-2,5-dienone(17),3-(3'-hydroxybutyl)-2,4,4-trimethylcyclohexa-2,5-dienone(18),4-isopropyl benzoic acid(19),p-cymen-7-yl β-D-gluco-pyranoside(20),acyclic 7-hydroxygeranic acid(21),7,7-dimethylbicyclo[3.1.1]hept-2-ene-l-carboxylic acid(22).And compounds 3-6,8,10-14,17-19,21 and 22 are isolated from this plant for the first time.The results of their anti-asthma activ-ities showed that compounds 11,14 could significantly inhibit the release of β-aminohexosidase.
万方数据
维普
