首页|HS-SPME-GC-MS分析'粉蕴'含笑不同开花时期花器官挥发性成分的变化规律

HS-SPME-GC-MS分析'粉蕴'含笑不同开花时期花器官挥发性成分的变化规律

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为探究'粉蕴'含笑(Michelia platypetala'Fenyun')花朵挥发性成分的时空变化规律,本研究以花蕾期、初开期和盛开期的花被片、雄蕊和雌蕊为试验材料,采用顶空固相微萃取与气质联用技术(HS-SPME-GC-MS)对其挥发性成分进行测定。结果表明,在'粉蕴'含笑花朵中共鉴定出93种挥发性成分,包括11大类,以萜烯类成分数量最多;并且,其总挥发性成分在花蕾期的花被片中释放量最高。在花被片、雄蕊和雌蕊中分别鉴定出73、86和86种挥发性成分,其中,相对含量大于2%的主要挥发性成分分别有8、10和11种,以2,6,10-三甲基十四烷、异十六(碳)烷、γ-杜松烯和β-异可烯为主。花被片中的特异性成分为乙酸桃金娘烯酯;雄蕊中的特异性成分为伞形花酮、木薄荷衍生物和反-2-辛醛;雌蕊中的特异性成分是别罗勒烯、十二烷、甲酸法呢酯、己醇和3,4-二氢-2H-吡喃。本研究进一步采用SIMCA14。1对鉴定出的挥发性成分进行主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)发现,从'粉蕴'含笑不同花器官中释放的挥发性成分能很好区分,其中有12种在不同花器官释放量有显著差异(VIP>1)。综上所述,'粉蕴'含笑的挥发性成分在花蕾期的花被片中含量最高,2,6,10-三甲基十四烷、异十六(碳)烷、γ-杜松烯和β-异可烯是'粉蕴'含笑开花过程中的主要挥发性成分。
HS-SPME-GC-MS analysis of the change pattern of volatile components in floral organs of Michelia platypetala'Fenyun'during different flowering stages
In order to investigate the spatiotemporal variations of volatile components in the flowers of Michelia platypetala'Fenyun',this study used the tepals,stamens,and pistils at the bud stage,initial flowering stage,and full flowering stage as experimental materials.The volatile components were determined by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS).The results showed that a total of 93 volatile components,belonging to 11 major groups,were identified in the flowers of M.platypetala'Fenyun',with sesquiterpenes being the most abundant.Furthermore,the highest release of total volatile components was observed in the tepals at the bud stage.A total of 73,86,and 86 volatile components were identified in the tepals,stamens,and pistils,respectively.Among them,there were 8,10,and 11 major volatile components with relative abundances greater than 2%.The main volatile components included 2,6,10-trimeth-yltetradecane,Isohecadecane,γ-cadinene,and β-isocomene.The specific components identified in the tepals were myrtenyl acetate,while the specific components in the stamens were umbellulone,prostantherol,derivative of and(E)-2-Octenal.In the pistils,the specific components included(E,Z)-2,6-Dimethylocta-2,4,6-triene,dodecane,farnesol formate,1-hexanol and 3,4-Dihydro-2H-pyran.In this study,further principal component analysis(PCA)and partial least squares-discriminant anal-ysis(PLS-DA)were performed on the identified volatile components using SIMCA 14.1.It was found that the volatile compo-nents released from different floral organs of M.platypetala'Fenyun'could be well distinguished.Among them,12 compo-nents showed significant differences in their release quantities among floral organs(VIP>1).In summary,the volatile com-ponents of M.platypetala'Fenyun'were found to be most abundant in the tepals at the bud stage.Among the identified com-pounds,2,6,10-trimethyltetradecane,isohecadecane,γ-cadinene,and β-isocomene were identified as the major volatile com-ponents during the flowering process of M.platypetala'Fenyun'.

Michelia platypetala'Fenyun'HS-SPME-GC-MSflower organsvolatile componentsPCAPLS-DA

郑超荣、胡莹冰、谭雨晴、雍玉冰、巢瑾、张哲、陈燕、金晓玲

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中南林业科技大学风景园林学院,长沙 410004

浙江广厦建设职业技术大学建筑工程学院,东阳 322100

湖南省茶业集团股份有限公司,长沙 410126

'粉蕴'含笑 HS-SPME-GC-MS 花器官 挥发性成分 PCA PLS-DA

林业公益性行业科研专项湖南省教育厅青年项目

20140471022B0259

2024

天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCD北大核心
影响因子:0.783
ISSN:1001-6880
年,卷(期):2024.36(3)
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