Study on UHPLC fingerprint and quantitative analysis of four components of Zingiber officinale from Yunnan
The fingerprints of 20 batches of Zingiber officinale from different origins in Yunnan were established by ultra-high performance liquid chromatography(UHPLC),and four gingerols were quantitatively analyzed.The UHPLC fingerprint of Z.officinale was established and 18 common chromatographic peaks of 20 batches of samples were analyzed by chemometrics,the samples could be divided into four groups by cluster analysis(CA),the chromatographic peaks were divided into three groups,the samples of Honghe and Wenshan were similar and clustered into two groups;and the samples of Qujing were clus-tered into two groups.The results of principal component analysis(PCA)showed that the differences between the samples of Honghe and Wenshan(Southeastern Yunnan)were relatively small,while and the differences between the samples of the two places and Qujing(Eastern Yunnan)were relatively large.The distribution of principal component scores and the loading of each variables were basically consistent with the classification results of CA;Orthogonal partial least square discriminant anal-ysis(OPLS-DA)could effectively distinguish southeastern Yunnan and eastern Yunnan,and eight differential markers were screened.The results of content determination showed that the content of Honghe and Wenshan Z.officinale samples were similar,and there were some differences between the two places and Qujing samples.The main differential components were 6-gingerol,8-gingerol and 6-gingerenol.This study can provide reference for the selection of origins and comprehensive evalua-tion of quality of medicinal Z.officinale.
万方数据
维普
