摘要
对瓦尼桑黄(Sanghuangporus vaninii)固体发酵产物的化学成分及生物活性进行研究.采用超声提取、柱层析以及半制备高效液相色谱等方法分离纯化,通过波谱数据鉴定了化合物的结构,并对所得单体化合物进行体外抗氧化活性、α-葡萄糖苷酶和黄嘌呤氧化酶抑制活性筛选.从瓦尼桑黄固体发酵产物中分离得到17个化合物,分别为(2Z,4E)-γ-ionylideneacetic acid(1)、phellinulin E(2)、phellinulin F(3)、phellinulin G(4)、phellinulin J(5)、phellinulin L(6)、phellinulin M(7)、phellinulin N(8)、elgonenes D(9)、咖啡酸(10)、hispidin(11)、3-hydroxyhispidin(12)、原儿茶酸(13)、4-(3-ethoxy-4-hydroxyphenyl)but-3-en-2-one(14)、丁子香酚(15)、亚油酸甲酯(16)、硬脂酸(17),其中化合物 1～9为首次从瓦尼桑黄中分离得到.活性测试结果显示化合物10～13表现出良好的抗氧化活性,对DPPH自由基的半数抑制浓度(IC50)分别为 16.45、37.52、82.71、50.46 μg/mL,对 ABTS 自由基的 IC50值分别为 1.43、2.26、34.55、21.60 μg/mL,对羟基自由基的IC50值分别为38.43、49.46、86.74、69.16 μg/mL.化合物10、11还对黄嘌呤氧化酶表现出良好的抑制作用,IC50值分别为28.73和44.80 μg/mL.化合物17具有一定的α-葡萄糖苷酶抑制活性,其IC50值 2.31 μg/mL.
Abstract
This paper investigated the chemical constituents and their bioactivities of solid fermentation products from Sang-huangporus vaninni.The chemical constituents were isolated by solution extraction,column chromatography and semi-prepara-tive high performance liquid chromatography,and their structures were elucidated by spectral analysis.All these isolated com-pounds were screened for antioxidant activities and inhibitory effects on α-glucosidase and xanthine oxidase in vitro.A total of 17 compounds were isolated from the extracts of solid fermented products from S.vaninni,and identified as(2Z,4E)-γ-io-nylideneacetic acid(1),phellinulin E(2),phellinulin F(3),phellinulin G(4),phellinulin J(5),phellinulin L(6),phellinulin M(7),phellinulin N(8),elgonenes D(9),caffeic acid(10),hispidin(11),3-hydroxyhispidin(12),protocat-echuic acid(13),4-(3-ethoxy-4-hydroxyphenyl)but-3-en-2-one(14),eugenol(15),methyl linoleate(16)and steraric acid(17).Among them,compounds 1-9 were isolated from S.vaninni for the first time.Compounds 10-13 showed good an-tioxidant activities in vitro.Their IC50 values for DPPH were respectively 16.45,37.52,82.71,50.46 μg/mL,for ABTS were 1.43,2.26,34.55,21.60 μg/mL and for hydroxyl radicals were 38.43,49.46,86.74,69.16 μg/mL.Compounds 10 and 11 also exhibited good inhibitory effects on xanthine oxidase,and their IC50 values were 28.73 μg/mL and 44.80 μg/mL,re-spectively.Compound 17 demonstrated certain inhibitory effect on α-glucosidase with IC50 2.31 μg/mL.
基金项目
天然产物研究与利用湖北省重点实验室(三峡大学)开放基金(2022NPRD10)
维普
万方数据