Chemical constituents from ethyl acetate fraction of Houttuynia cordata and their anti-plant pathogens activity
Ten compounds were separated and purified from ethyl acetate fraction derived from 95％EtOH extract of Hout-tuynia cordata by silica gel,macroporous resin,Sephadex LH20,MCI resin,thin-layer chromatography and high performance liquid chromatography.Their structures were identified on the basis of mass spectrometry(MS),nuclear magnetic resonance and comparison with the data in reported literatures.As a result,ten compounds were identified as dehydrovomifoliol(1),5,6-epoxy-3-hydroxy-7-megastigmen-9-one(2),loliolide(3),pubinernoid A(4),quercitrin(5),quercetin(6),afzelin(7),4-methoxybenzene-1,2-diol(8),4-hydroxybenzaldehyde(9),benzoic acid(10).Compounds 1-4 were norsesquiterpe-noids isolated from Houttuynia cordata for the first time.All the compounds were screened for their anti-plant pathogens activ-ities,and at the concentration of 40 mg/mL,the inhibition rates of compounds 5-7 ranged from 38％to 50％against Fusarium graminearum,Fulria fulva,Botrytis cinerea,Mycosphaerlla melonis and Fusarium oxysporum f.sp.vasinfectum.
Houttuynia cordataisolation and identificationnorsesquiterpenoidsanti-plant pathogens activity
万方数据
维普
