The objective of this study was to examine the impact of salvianolic acid B(Sal B)on liver injury in mice,as well as its effects on intestinal flora and short-chain fatty acids(SCFAs).To establish a mouse liver injury model,a subcutaneous injection of 20％CCl4 solution was administered.At the same time,mice in treatment groups were subjected to Sal B and the positive drug 2-deoxy-D-glucose gavage intervention,respectively.After the administration,the pathological changes of liver tissue were observed by hematoxylin-eosin(HE),Masson,and Sirius red staining,and the levels of serum alanine aminotrans-ferase(ALT)and aspartate aminotransferase(AST)were detected by biochemical kits.16S rRNA sequencing technology was used to detect the changes of intestinal microbiota in mice.Additionally,the contents of SCFAs in mice feces were deter-mined through gas chromatography.The results indicate that Sal B has a significant effect on reducing serum ALT and AST levels(P＜0.001)and mitigating liver pathological damage.Moreover,Sal B was revealed to partly restore the composition of intestinal flora in mice with liver injury,as evidenced by the sequencing data.Furthermore,the administration of Sal B re-sulted in a significant increase in the concentrations of isobutyric acid,isovaleric acid,propionate acid,and valeric acid in the feces from mice with liver injury(P＜0.001).Consequently,the above results could conclude that Sal B possesses the abili-ty to alleviate liver injury induced by CCl4 in mice.The mechanism is potentially related to its regulation of the balance of in-testinal flora and the restoration of the contents of SCFAs.
salvianolic acid Bliver injuryintestinal florashort-chain fatty acids
维普
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