Antidiabetic and antioxidant activities of ethanol extracts from Moutan Cortex
This study aimed to fully utilize the resources of Moutan Cortex,40％,70％and 95％ethanol solutions were used to extract the active components of Moutan Cortex by ultrasound,and 40％,70％and 95％ethanol extracts(MC40,MC70 and MC95)were obtained,respectively.The contents of total phenols and total flavonoids,as well as the antioxidant capacity and anti-diabetic activity in vitro of the three extracts were compared.Finally,the zebrafish model in vivo was used to evaluate the hypoglycemic and peripheral motor neuroprotective activities of the extracts.The results show that MC70 had the highest con-tent of total phenol(59.17 mg GAE/g,gallic acid equivalent)and total flavone(82.59 mg QUE/g,quercetin equivalent).Meanwhile,MC70 exhibited the strongest antioxidant activity,with IC50 values of 34.20 µg/mL and 16.30 μg/m for DPPH and ABTS free radical scavenging capacities,respectively,and MC40 showed the weakest antioxidant activity.In addition,MC95 exhibited the highest inhibition rate of glycosylation(95.92％),followed by MC70(81.44％).The experimental results of the hyperglycemic zebrafish model showed that MC70 treatment resulted in a 52％decrease in blood glucose levels and a 56％re-duction in peripheral motor nerve injury,compared with the model group.The results suggest that 70％ethanol was the suit-able solvent for extracting antioxidant and hypoglycemic components from Moutan Cortex,and the extract has good antioxidant and anti-diabetic potential,providing a theoretical basis for the subsequent high-value utilization of the peel.
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