Study on the improvement effect and mechanism of Ganoderma lucidum polysaccharides on diabetic nephropathy in mice
The study investigated the effects of Ganoderma lucidum polysaccharide(GLP)on diabetic nephropathy(DN)in mice.After successfully establishing a DN mouse model,GLP intervention was administered.The fasting blood glucose and 24-hour urinary protein content of the mice were measured,and the levels of blood urea nitrogen(BUN)and serum creatinine(Scr)were determined.Eosin and Masson's trichrome staining were used to observe the renal pathological morphology.En-zyme-linked immunosorbent assay was employed to measure the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and interleukin-10(IL-10).Western blot was utilized to detect the expression of receptor for advanced glycation end-products(RAGE),collagen type Ⅳ(COL-Ⅳ),and inducible nitric oxide synthase(iNOS).Mice in the DN group exhibited increased levels of fasting blood glucose,24-hour urinary protein,BUN,and Scr as well as elevated levels of TNF-α and IL-6 in renal tissue(P＜0.05),and decreased levels of serum IL-10(P＜0.05).The expression of RAGE,COL-Ⅳ,and iNOS proteins was also increased(P＜0.05).In contrast,mice in the treatment group showed improvements in pathological dam-age and fibrosis,with quantitative indicators showing significant amelioration.GLP could ameliorate DN in mice,and the mechanism is associated with the inhibition of the RAGE signaling pathway.
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