摘要
研究野菊Chrysanthemum indicum L.地上部分中萜类成分及其抗炎活性.采用MCI、ODS、SephadexLH-20凝胶柱色谱和pre-HPLC等方法分离纯化,再通过对其波谱数据分析鉴定化合物的结构.从野菊二氯甲烷提取物中分离纯化得到 6 个单体化合物,分别是 chrygermalide A(1)、3β-hydroxy-oxo-7α,11β-germacra-4Z,10(14)-diene-12,6α-ol-ide(2)、deacetylherbolide A(3)、9β-hydroxy costunolide(4)、3β-hydroxycostunolide(5)、3β-hydroxy-11α,13-dihydrocos-tunolide(6).其中化合物1为新化合物,2～6为首次从该属植物中分离得到.采用LPS诱导RAW 264.7巨噬细胞的炎症模型测试了化合物1～6的抗炎活性,其NO抑制率IC50值在17.9～33.9μmol/L之间.
Abstract
To study the chemical constituents of the aboveground of Chrysanthemum indicum L.All compounds were separated and purified by MCI,ODS,SephadexLH-20 gel column chromatography and pre-HPLC,and the structures of them were identi-fied by analyzing of their spectroscopic data.Compounds 1-6 were isolated from the dichloromethane extract of C.indicum,which were identified as chrygermalide A(1),3β-hydroxy-oxo-7α,11β-germacra-4Z,10(14)-diene-12,6α-olide(2),deacetylherbolide A(3),9β-hydroxy costunolide(4),3β-hydroxycostunolide(5),3β-hydroxy-11α,13-dihydrocos-tunolide(6).Compound 1 is a new compound,and 2-6 are isolated from this genus for the first time.The anti-inflammatory activity of compounds 1-6 were tested in LPS-induced RAW 264.7 macrophage cells,and the IC50 values of their NO release inhibition were 17.9-33.9 μmol/L.
基金项目
国家自然科学基金(82003606)
河南省高校科技创新团队支持计划(24IRTSTHN039)
国家科技期刊平台
NSTL
万方数据