首页|常山胡柚果皮精油提取工艺模型筛选优化、化学成分及抗氧化活性研究

常山胡柚果皮精油提取工艺模型筛选优化、化学成分及抗氧化活性研究

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研究常山胡柚果皮(Citrus × aurantium Changshan-huyou pericarp,CP)精油化学成分,并对提取条件进行优化.采用水蒸气蒸馏法提取CP精油,利用气相色谱-质谱法进行分析,通过单因素试验考察不同提取条件对提取率的影响,并利用响应面、人工神经网络进行工艺优化.结果表明,CP精油中主要成分为D-柠檬烯;最佳工艺条件:粒径24目、蒸馏水浸泡1 h、液固比34.86 mL/g、提取温度300.00 ℃、提取时间166.21 min,在此条件下,CP精油提取率为0.82％,响应面与人工神经网络相结合,可有效地利用已有数据对目标寻优.同时CP精油对DPPH自由基有明显的清除作用(IC50值为65.01 mg/mL),表明CP精油具有一定的抗氧化活性.
Screening and optimization of essential oil extraction process model of Citrus × aurantium Changshan-huyou pericarp and its chemical constituents and antioxidant activity
This study aims to study the chemical constituent of essential oil from Citrus × aurantium Changshan-huyou peri-carp(CP)and optimize the extraction conditions.The essential oil constituents in CP were extracted by steam distillation,and GC-MS was applied to qualitatively analyze the chemical constituents of essential oil in CP.The single-factor test was used to investigate the effect of different extraction conditions on the extraction yield of essential oil.Response surface methodology optimization and artificial neural network were used to screen the optimal extraction process of essential oil.The results showed that the main constituents in CP essential oil was D-limonene.The optimal technological parameters were as follows:particle size of 24 mesh,distilled water soaking for 1 h,liquid-solid ratio 34.86 mL/g,extraction temperature 300.00 ℃,ex-traction time 166.21 min,under these conditions,the extraction yield of the essential oil was 0.82％.The combination of re-sponse surface methodology and artificial neural network can effectively optimize the target by using the existing data.Mean-while,CP essential oil possessed significant scavenging ability on DPPH free radical(IC50 value=65.01 mg/mL),indica-ting that CP essential oil had a certain degree of antioxidant activity.

Citrus × aurantium Changshan-huyouessential oilsteam distillationresponse surface methodology optimiza-tionartificial neural network

江丽洁、温乐乐、石佳艺、邓雅慧、刘巨钊、袁强、崔琦

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浙江中医药大学药学院,杭州 311402

常山胡柚 精油 水蒸气蒸馏法 响应面优化 人工神经网络

国家自然科学基金浙江省自然科学基金浙江中医药大学科研项目浙江省大学生新苗人才计划

82204552LQ24H2800092022JKZKTS102023R410043

2024

10.16333/j.1001-6880.2024.7.001

天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCD北大核心
影响因子：0.783
ISSN：1001-6880
年,卷(期)：2024.36(7)