Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp.GTD4C-2 and their antioxidant activity
The optimization of intracellular polysaccharides(IPSs)extraction from Chlorella sp.GTD4C-2 was performed u-sing response surface methodology based on a central composite design.The optimal conditions for extraction were determined as follows:an extraction time of 1 h,an extraction temperature of 80 ℃,and a solid-to-liquid ratio of 1:25(g/mL).Subse-quently,the extracted IPSs were further purified through ion exchange chromatography.Structural analysis was carried out u-sing high-performance gel permeation chromatography,gas chromatography-mass spectrometry,and Fourier-transform infrared spectroscopy.Additionally,the DPPH radical scavenging activity and hydroxyl radical scavenging capacity of the IPSs were e-valuated.The results revealed two stable molecular weight acidic polysaccharides:IPS-2(53.1 kDa)and IPS-3(25.6 kDa),both containing glucose and galactose as monosaccharide components.The sulfate content was found to be(5.69±0.11)％for IPS-2 and(8.46±0.03)％for IPS-3.In vitro antioxidant activity showed that vitamin C exhibited the highest antioxidant capacity followed by the crude extract of IPSs,then IPS-3,and finally IPS-2.In conclusion,polysaccharides extracted from Chlorella sp.GTD4C-2 demonstrated significant antioxidant activity with stronger antioxidative properties observed in the crude extract compared to isolated compounds(IPS-2 and IPS-3).These findings suggest promising applications for Chlorella sp.GTD4C-2 polysaccharides in various fields such as food products,pharmaceuticals,and health supplements.
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