摘要
为研究肉桂(Cinnamomum cassia Presl)叶的化学成分,采用大孔树脂、ODS、硅胶柱色谱及半制备高效液相等色谱分离技术对肉桂叶正丁醇萃取部位进行分离纯化.共从中分离得到8个化合物,根据其理化性质及波谱数据进行结构鉴定,分别为(7S,8S)-4,9,7'-trihydroxy-3,3',5'-trimethoxy-8-O-4'-neolignan 7-O-β-D-glucopyranoside(1)、蚱蜢酮(2)、柑橘苷 A(3)、loliolide-β-D-glucopyranoside(4)、methyl dihydromelilotoside(5)、methyl 2-phenylpropanoate-2-O-β-D-apiofuranosyl-(1 →6)-O-β-D-glucopyranoside(6)、2,3-dihydroxy-1-(4-hydroxyl-3,5-dimethoxyphenyl)-1-propanone(7)、4-O-(2-hydroxy-1-hydroxymethylethyl)-dihydroconiferyl alcohol(8).化合物 1 为新的苯丙素葡萄糖苷,化合物 3～5、7、8为首次从该植物中分离得到.采用HepG2细胞胰岛素抵抗模型对化合物1～8进行体外降血糖活性筛选,结果显示,在10.0 μmol/L浓度下,化合物2、3、7和8可以增加胰岛素抵抗HepG2细胞的葡萄糖消耗量.
Abstract
This study aims to investigate the chemical constituents of the leaves of Cinnamomum cassia.The extract of the leaves of C.cassia was separated and purified utilizing chromatographic techniques including macroporous resin,ODS,silica gel column chromatography and semi-preparative high performance liquid phase.Eight compounds were isolated from the n-butanol fraction of the leaves of C.cassia,and their structures were identified as(7S,8S)-4,9,7'-trihydroxy-3,3',5'-trime-thoxy-8-O-4'-neolignan7-O-β-D-glucopyranoside(1),grasshopper ketone(2),citroside A(3),loliolide-β-D-glucopyrano-side(4),methyl dihydromelilotoside(5),methyl 2-phenylpropanoate-2-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(6),2,3-dihydroxy-1-(4-hydroxyl-3,5-dimethoxyphenyl)-1-propanone(7),4-O-(2-hydroxy-1-hydroxymethylethyl)-di-hydroconiferyl alcohol(8).Among them,compound 1 is a new phenylpropanoid glucoside,and compounds 3-5,7,and 8 were isolated from the plant for the first time.Compounds 2,3,7 and 8 could significantly increase the glucose consumption in insu-lin-resistant HepG2 cells at a concentration of 10.0 mol/L.
基金项目
国家自然科学基金面上项目(22177027)
河南省高校科技创新团队支持计划(24IRTSTHN039)
河南省科技研发计划联合基金(优势学科培育类)(232301420067)
维普
万方数据