研究"十大皖药"品种——九华黄精(Polygonatum cyrtonema Hua in Jiuhua Mountain)块状根茎的化学成分及其抗炎活性.综合采用硅胶柱色谱、MCI柱色谱、Sephadex LH-20凝胶柱色谱及半制备高效液相色谱等方法从九华黄精生药材的85％乙醇提取物中共分离得到16个化合物,并通过1 H NMR、13C NMR、HR-ESI-MS技术鉴定了化合物结构,分别为 polygodoside H(1)、polygonatumoside G(2)、25(S)-funkioside B(3)、typaspidoside A(4)、芦丁(5)、木犀草素-7-O-芸香糖苷(6)、山柰酚-7-O-β-D-吡喃葡萄糖苷(7)、槲皮素-3-O-β-D-吡喃葡萄糖苷(8)、芹菜素-7-O-β-D-葡萄糖苷(9)、落叶松脂醇-4-O-β-D-葡萄糖苷(10)、5-O-咖啡酰奎宁酸甲酯(11)、4-O-咖啡酰奎宁酸甲酯(12)、3,5-O-二咖啡酰奎宁酸甲酯(13)、3,4-O-二咖啡酰基奎宁酸甲酯(14)、4,5-O-二咖啡酰基奎宁酸甲酯(15)、对羟基肉桂酸甲酯(16),所有化合物均是首次从九华黄精中分离得到.活性筛选结果显示化合物1～3、5～8均能够有效抑制LPS诱导的RAW 264.7细胞释放NO,且无细胞毒作用,IC50值范围为8.28～41.85 μmol/L,表现出一定程度的抗炎活性.
Chemical constituents from Polygonatum cyrtonema Hua in Jiuhua Mountain and their anti-inflammatory activity
This study aims to investigate the chemical constituents from the massive rhizomes of Polygonatum cyrtonema Hua in Jiuhua Mountain,together with their inflammatory activities.Sixteen compounds were isolated and purified from the 85％ethanol extract of the title plant by using systematic separation methods,including silica gel column chromatography,MCI col-umn chromatography,Sephadex LH-20 gel column chromatography and semi-preparative liquid chromatography.Their struc-tures were identified as polygodoside H(1),polygonatumoside G(2),25(S)-funkioside B(3),typaspidoside A(4),rutin(5),luteolin-7-O-rutinoside(6),kaempferol-7-O-β-D-glucoside(7),quercetin-3-O-β-D-glucopyranoside(8),apigenin-7-O-β-D-glucoside(9),lariciresinol glycoside(10),5-O-caffeoylquinic acid methyl ester(11),4-O-caffeoylquinic acid methyl ester(12),3,5-O-dicaffeoylquinic acid methyl ester(13),3,4-O-dicaffeoylquinic acid methyl ester(14),4,5-O-dicaf-feoylquinic acid methyl ester(15),trans-p-coumaric acid methyl ester(16)by 1H NMR,13C NMR and HR-ESI-MS.All the compounds are isolated from this plant for the first time.Biologically,all compounds were subjected to evaluate their anti-in-flammatory activities via inhibiting NO production in LPS-stimulated RAW 264.7 cells in vitro.The results indicated that compounds 1-3,5-8 showed a moderate inhibitory effect against NO production with IC50 values of 8.28-41.85 µmol/L and without cytotoxicity against the cells,showing a certain degree of anti-inflammatory activity.
Polygonatum cyrtonema Hua in Jiuhua Mountainchemical constituentsstructure identificationanti-inflammatory activity
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维普
