This study aims to investigate the chemical constituents from Nelumbinis Plumula and their protective effects on cardiac muscle cell.Fourteen compounds were isolated and purified from 80％ethanol extract by various column chromatogra-phy techniques,and identified by 1H NMR,13C NMR and other spectroscopic techniques.The compounds were screened for cardiomyocyte protective activity.Fourteen compounds were identified as naringin(1),kaempferol-3-O-β-D-rutinoside(2),apigenin-6-C-β-L-arabinopyranosyl-8-C-β-D-glucopyranoside(3),luteolin-7-O-β-D-neohesperidoside(4),luteolin-3'-O-β-D-glucopyranoside(5),apigenin-7-O-β-D-glucopyranoside(6),hesperidin(7),isoschaftoside(8),pinoresinol-4-O-β-D-glu-copyranoside(9),p-coumaric acid-4-O-β-D-glucopyranoside(10),p-coumaric acid(11),phenethyl-6-O-α-L-arabinfurano-syl-β-D-glucopyranoside(12),icariside D1(13),icariside F2(14).Compounds 1-6,9,and 11-14 were firstly isolated from Nelumbinis Plumula.Flavonoids compounds 1-8 showed significant protective effects against hypoxia/reoxygenation injury in HL-1 cardiomyocytes,as well as promoting effects on STAT3 expression in HL-1-STAT3-Luc cells in dose-dependent manner.
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