摘要
研究桂林会仙湿地来源真菌Talaromyces sp.W21的化学成分及其抗炎活性.综合运用多种柱色谱、现代波谱技术和理化性质对Talaromyces sp.W21发酵提取物中的化学成分进行分离、纯化和结构鉴定,并从中分离得到10个化合物,分别鉴定为 cytospone K(1)、(3S,4R,5R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2)、lasdiplactone(3)、leptosphaerone C(4)、annularin A(5)、annularin K(6)、cladobotrin V(7)、2-(2'S-hydroxypropyl)-5-methyl-7-hydroxy-chromone(8)、bacillisporin A(9)、bacillisporin B(10).其中,化合物1为新的α-吡喃酮类化合物,化合物2～8为首次从Talaromyces sp.中分离获得.抗炎活性实验结果表明化合物9和10对LPS诱导的RAW 264.7释放NO具有一定的抑制作用,其 IC50分别为 38.90±0.79、35.02±1.03 μmol/L.
Abstract
This study aims to investigate the chemical constituents and anti-inflammatory activity of Talaromyces sp.W21 from the Huixian wetland in Guilin.A new α-pyrone derivative,namely cytospone K(1),together with nine known compounds,namely(3S,4R,5R)-4-hydroxymethyl-3,5-dimethyldihydro-2-furanone(2),lasdiplactone(3),leptosphaerone C(4),annu-larin A(5),annularin K(6),cladobotrin V(7),2-(2'S-hydroxypropyl)-5-methyl-7-hydroxychromone(8),bacillisporin A(9)and bacillisporin B(10)were isolated and purified from cultures of Talaromyces sp.W21 by using various column chromatography.Their structures were identified by modern spectroscopic techniques and physicochemical properties.Com-pound 1 was a new α-pyranone derivative,and compounds 2-8 were isolated from the Talaromyces sp.for the first time.The anti-inflammatory activity assay showed that compounds 9 and 10 have a moderate inhibition of NO production induced by LPS in RAW 264.7 cells,with IC50 values of 38.90±0.79 and 35.02±1.03 µmol/L respectively.
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