建立搓条和未搓条红芪样品HPLC指纹图谱,结合多成分定量,比较搓条和未搓条样品的差异.采用Angi-lent C18色谱柱(4.6 mm × 250 mm,5 µm),流动相为0.1％磷酸水溶液-乙腈(梯度洗脱),流速1.0mL/min,柱温30℃,建立14批搓条和未搓条样品指纹图谱,通过相似度评价和化学计量学分析,研究不同红芪样品的差异性,测定毛蕊异黄酮、芒柄花素、总多糖、总黄酮等成分的含量.结果显示,搓条和未搓条样品均标定25个共有峰,指认了其中7个成分,分别为腺苷、香草酸、毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素、美迪紫檀素.聚类分析、主成分分析均能很好地区分搓条和未搓条样品,正交偏最小二乘-判别分析共找到12个差异性标志物,差异显著性排序分别为峰25＞峰23＞峰14＞芒柄花素＞毛蕊异黄酮苷＞毛蕊异黄酮＞峰18＞峰19＞峰15＞芒柄花苷＞峰4＞香草酸.含量测定结果显示,红芪搓条样品中毛蕊异黄酮、芒柄花素、浸出物、总多糖含量明显高于未搓条样品,毛蕊异黄酮苷、芒柄花苷、香草酸、总黄酮含量明显低于未搓条样品.该研究为红芪质量和搓条加工提供数据参考.
Comparative study on rubbed and unrubbed Hedysari Radix based on multi-component determination
To establish the HPLC fingerprints of rubbed and unrubbed Hedysari Radix samples,the differences between rubbed and unrubbed samples were compared with multi-component quantification.The chromatographic column was Angilent C18(4.6 mm × 250 mm,5 µm).The flow rate was 1.0 mL/min,and the mobile phase consisted of 0.1％phosphoric acid solution-acetonitrile(gradient elution).The column temperature was 30 ℃.The fingerprints of 14 batches of rubbed and un-rubbed samples were established,through similarity evaluation and chemometric analysis,and the difference between rubbed and unrubbed samples was studied subsequently.The contents of components such as calycosin,formononetin,total polysac-charides,and total flavonoids were determined.The results showed that there were 25 common peaks in the fingerprint of rubbed and unrubbed samples.Seven components were identified as adenosine,vanillic acid,calycosin-7-glucoside,ononin,calycosin,formononetin,medicarpin.Both hierarchical clustering analysis and principal component analysis can be distin-guished between rubbed and unrubbed samples.Orthogonal partial least squares-discriminant analysis found twelve compo-nents of difference,and the order of significance of difference was peak 25＞peak 23＞peak 14＞formononetin＞calycosin-7-glucoside＞calycosin＞peak 18＞peak 19＞peak 15＞ononin＞peak 4＞vanillic acid.The results of content determina-tion showed that the content of calycosin,formononetin,total polysaccharide,and extract in rubbed samples was significantly higher than in the unrubbed ones.In contrast,the contents of calycosin-7-glucoside,ononin,vanillic acid,and total flavonoids in rubbed samples were significantly lower than in the unrubbed ones.The study can provide data reference for quality and rubbing processing of Hedysari Radix.
万方数据
维普
