This study aims to explore the role and mechanism of Cordyceps polysaccharides(CSP)in improving macrophage efferocytosis function based on peroxisome proliferator-activated receptor γ(PPAR-γ).The CSP was extracted by hydro-alco-hol precipitation.The molecular weight distribution,monosaccharide composition and methylation products of CSP were detec-ted by high performance liquid gel chromatography,PMP-high performance liquid chromatography and methylation analysis,respectively.The effects of CSP on mouse macrophage cell viability,efferocytosis-related genes and efferocytosis rate were de-termined by CCK-8 assay,real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and flow cytometry,re-spectively.The effect of CSP on PPAR-γ expression and nuclear localization was observed by immunofluorescence.Si-RNA was then used to knock down PPAR-γ to verify the possible target genes and signaling pathways of CSP.The results showed that the CSP contained 72.9％polysaccharides,with a molecular weight distribution of 21.9,2 100 kDa and more than 50000 kDa.The CSP was mainly composed of glucose,galactose and mannose,and it contains six main glycosidic bonds.Com-pared with the model group,CSP increased the expression of efferocytosis-related genes TAM receptor tyrosine kinase(Tyro3/Axl/Mer receptor tyrosine kinase,TAM),milk fat globule-epidermal growth factor 8(Mfge8)and the efferocytosis rate of macrophages(P＜0.05).Meanwhile,CSP promoted the protein expression of PPAR-γ(P＜0.001)with nuclear transloca-tion.In addition,the ability of CSP to promote the expression of macrophage efferocytosis-related genes was significantly af-fected after knockdown of PPAR-γ(P＜0.05).Our study suggests that CSP may enhance oxidized low-density lipoprotein(ox-LDL)-induced macrophage efferocytosis through activating PPAR-γ signaling pathway.
维普
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