This study aims to investigate the taxane diterpenoid constituents of Taxus chinensis var.mairei from Yunnan Prov-ince and their myeloma cytotoxic activities.Compounds were isolated and purified from the extract of the leaves and branches of Taxus chinensis var.mairei by using normal-phase,reversed-phase and size exclusion chromatography,and their structures were identified by NMR spectroscopic methods.The isolated compounds were screened for activity against NEK2 over expres-sion myeloma cell proliferation using a CCK-8 assay.Sixteen taxane diterpenoids were obtained and identified as 13-cin-namoyloxy-1-dehydroxy baccatin Ⅳ(1),taxusin(2),5α-cinnamoyloxy-9α,10β,13α-triacetoxytaxa-4(20),11-diene(3),7-deacetoxy taxinine J(4),hongdoushan A(5),11-diene-2α,5α,9α,10β,13β-pentol-2α,9α,10β,13α-tetraacetate-5α-cin-namate(6),taxezopidine H(7),taxinine B(8),taxuyunnanine C(9),1-deoxybaccatin Ⅵ(10),19-hydroxybaccatin Ⅲ(11),baccatin Ⅲ(12),7,9,13-trideacetyl baccatin Ⅵ(13),10-deacetylbaccatin Ⅲ(14),taxol(15),7-epi-taxol(16).Compound 1 was a new taxane diterpenoid,while compounds 5 and 13 were isolated from Taxus chinensis var.mairei for the first time.The results indicated that compounds 2,6,7 and 11 had notable inhibitory activity on NEK2 over expression myelo-ma cell proliferation(IC50=24.0,23.5,26.1,18.3μmol/L),and compound 16(IC50=0.6 μmol/L)had a significant effect.
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