Chemical constituents from EtOAc extract of Pinelliae Rhizoma and their anti-inflammatory activity
This study aims to invstigate the chemical constituents from Pinelliae Rhizoma and their anti-inflammatory activity.Twenty-six compounds were isolated and purified from the ethyl acetate extract of Pinelliae Rhizoma silica gel,MCI,ODS and Toyopearl HW-40C column chromatography and semi-preparative HPLC methods.Their chemical structures were elucidated by physiochemical properties and spectral data as cyclo-(L-Pro-8-hydroxy-D-Ile)(1),cyclo-(L-Pro-L-Leu)(2),cyclo-(L-Pro-L-Phe)(3),cyclo-(L-Pro-L-Val)(4),adenosine(5),5'-S-methyl-5'-thioadenosine(6),2'-methoxyadenosine(7),adenine(8),1,8,15-triazecycloterpine-2,9,16-trione(9),1,8,15,22-tetraazacyclooctacosane-2,9,16,23-tetraketone(10),1,8,15,22,29-pentaaza-pentadecane-2,9,16,23,30-pentanone(11),diethylene glycol azelate(12),glycerol monop-almitate(13),glycerin monolinoleate(14),9,12-octadecadienoic acid N-(2-hydroxyethyl)(15),2-butoxyethyl linolenate(16),(2S)-1-O-(9Z,12Z-octadecandienyl)-3-O-β-galactosyl glycerol(17),β-sitosteryl-3β-glucopyranoside-6'-O-palmitate(18),bis(2-ethylhexyl)phthalate(19),lariciresinol(20),lariciresinol-9-O-β-glucopyranoside(21),(+)-epipinoresinol-4'-O-β-glucopyranoside(22),(+)-isolariciresinol-9'-O-β-glucopyranoside(23),(+)-isolariciresinol-9-O-β-glucopyrano-side(24),vanillic acid(25),and(E)-p-coumaroyl alcohol(26).Compounds 1,3,4,8-12,15,16,19 and 23 were isolated from Pinelliae Rhizoma for the first time.Some of isolated compounds were further evaluated for their anti-inflammatory activi-ty in lipopolysaccharide(LPS)-induced mouse macrophage(RAW 264.7)cells.The results showed that compounds 2,3,11,16 and 21 had inhibitory effects on NO release,and compound 16 had stronger NO inhibition with IC50 values of 10.47±0.89 μmol/L.
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