Flavonoids from Rhododendron nivale and their antioxidant and anti-inflammatory activities
This study investigates the flavonoid compounds in the extracts of R.nivale and their antioxidant and anti-inflamma-tory activities.Various chromatographic separation techniques such as silica gel column chromatography,Sephadex LH-20 col-umn chromatography,and semi-preparative high-performance liquid chromatography(HPLC)were used to separate and puri-fy the ethyl acetate extracts of R.nivale.Using NMR,IR,and HR-ESI-MS,eight flavonoid compounds were identified,inclu-ding a new one,3',8-dimethoxyquercetin-3-O-α-L-arabinofuranoside(1),and seven known compounds:morin-3-O-β-L-lyxo-side(2),(2R,3R)-taxifolin-3-O-arabinoside(3),epicatechin(4),(-)-epigallocatechin(5),(-)-catechin(6),dihydro-myricetin(7),dihydroquercetin(8).Their antioxidant and anti-inflammatory activities were measured using a H2O2-induced oxidative damage model and a lipopolysaccharide-induced nitric oxide production model,respectively.Compounds 2,4 and 8 increased the viability of H2O2-damaged SH-SY5Y cells from(44.0±2.3)％to(80.6±0.4)％,(78.4±1.6)％and(81.9±1.7)％,respectively.Compound 8 also exhibited anti-inflammatory activity with an IC50 value of 37.7 μmol/L.These results demonstrate that the flavonoid compounds in R.nivale have potential as lead compounds for developing antioxi-dant and anti-inflammatory agents.
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