Chemical constituents and anti-inflammatory activity of Pimpinella candolleana(Ⅱ)
This study aims to explore the chemical constituents and anti-inflammatory activities of extracts from Pimpinella candolleana.The 70％ethanol extract from P.candolleana were isolated by different chromatographic procedures including silica gel,ODS gel,D101 macroporous resin and MCI resin column.The structures of the compounds were identified by com-paring the spectral data with the literature.Their potential anti-inflammatory effects were evaluated on murine macrophage cell line(RAW 264.7)stimulated by lipopolysaccharide(LPS).Twenty-five compounds were isolated and identified as 1-hy-droxy-2,3,4,7-tetramethoxyxanthone(1),1-hydroxy-2,3,4,6-tetramethoxyxanthone(2),bellidifolin(3),desmethylbellidifo-lin(4),swertianolin(5),swertiajaponin(6),isoswertiajaponin(7),luteolin-7-O-β-D-rutinoside(8),luteolin-7-O-β-D-gluco-pyranosyl-(1→6)-[6'''-O-caffeoyl]-β-D-glucopyranoside(9),luteolin-4'-O-β-D-glucopyranoside(10),luteolin-6-C-β-L-fu-coside(11),orientin(12),isoorientin(13),quercetin-3-O-β-D-glucopyranoside(14),apigenin(15),quercetin-3-O-β-D-(6"-caffeoylgalactoside)(16),tricin-7-O-β-D-glucopyranoside(17),puerarin(18),vitexin(19),ombuin-3-O-β-D-glucopyrano-side(20),ombuin-3-O-β-D-galacopyranoside(21),rhamnetin-3-O-β-D-galactopyranoside(22),rhamnetin-3-O-β-D-glucopyr-anoside(23),isorhamnetin-3-O-β-D-glucopyranoside(24),chrysoeriol-7-O-β-D-glucopyranoside(25).Compounds 13-15 were firstly isolated from this plant.Furthermore,compounds 1-12,16-25 were isolated from the genus Pimpinella for the first time.The results of bioassay showed that compounds 1-3,6-10,12-15,17-20,23,25 exhibited different degrees of inhibitory effect against NO production in LPS-stimulated RAW 264.7 cells and displayed potential anti-inflammatory activity.
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