Anti-inflammatory activity and mechanism of an oleanane triterpenoid saponin from Camellia oleifera fruit shell
In this study,lipopolysaccharide(LPS)-induced murine peritoneal macrophage RAW 264.7 cells were used as the inflammation model,and Griess assay,enzyme-linked immunosorbent assay(ELISA),Western blotting and immunofluores-cence techniques were used to evaluate the anti-inflammatory activity of 3-O-β-D-glucopyranosyl(1"→6')α-L-rhamnosyl 27-hydroxy oleanolic acid-28-O-β-D-glucopyranoside(OA)from Camellia oleifera fruit shell.And control group,LPS group and OA group(5,10,20 and 40 μmol/L)were set up.The results showed that OA inhibited the secretion of nitric oxide(NO),prostaglandin E2(PGE2),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)at different concentrations,and de-creased the protein expression of NOD-like receptor family,pyrin domain containing 3(NLRP3)and cysteine aspartate prote-ase 1(Caspase 1)at 20 or 40 μmol/L concentrations in LPS-induced RAW 264.7 cells.Moreover,OA significantly down-regulated the phosphorylation levels of extracellular signal regulated kinase(ERK)and p38 protein(p38)at 1 and 6 h,and had inhibitory activity on the phosphorylation levels of p38 and Jun N-terminal kinase(JNK)at 2 h.Furthermore,OA at dif-ferent concentrations significantly blocked nuclear translocation of nuclear factor-κB(NF-κB)in LPS-induced RAW 264.7 cells after 6 h treatment.These results revealed the anti-inflammatory activity of OA,and provided a scientific basis for the targeted development of C.oleifera fruit shell resources and OA based active products.
Camellia oleifera fruit shelltriterpenoid saponinanti-inflammatory activityRAW 264.7lipopolysaccharide
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