Study on the quantitative analysis and quality difference evaluation of Eupatorii Herba from different producing areas
This study aims to establish a method for simultaneous determination of 11 components of Eupatorii Herba from dif-ferent producing areas,and to evaluate its quality difference by chemical pattern recognition and grey relational analysis(GRA).The 18 batches of Eupatorii Herba samples from eight provinces were collected.A high performance liquid chroma-tography method was used to simultaneous determine contents of rutoside,isorhamnetin-3-O-rutinoside,luteolin,quercetin,tar-axasterol,β-sitosterol,stigmasterol,fumaric acid,succinic acid,1,2-benzopyrone and eupatolide,and the multi-components quantitative control mode of Eupatorii Herba was established.Chemical pattern recognition and GRA were used to establish the quality difference evaluation model of Eupatorii Herba,and the overall quality was evaluated comprehensively.Under the constructed chromatographic conditions,the methodological validation of the external standard method meets the requirements of the pharmacopoeia;There was basically no difference in the detection results between(quantitative analysis of multicompo-nents by single-marker,QAMS)method and external standard method.The chemical recognition pattern results indicated that 18 batches of samples were clustered into three categories;rutoside,stigmasterol,luteolin,quercetin,1,2-benzopyrone and β-sitosterol may be the main potential markers affecting the quality of Eupatorii Herba.The results of GRA showed that the rela-tive correlation of 18 batches of Eupatorii Herba were 0.418 5,0.477 9,0.544 6,0.503 5,0.509 9,0.544 0,0.486 8,0.3388,0.354 8,0.360 8,0.329 8,0.291 0,0.618 6,0.627 3,0.644 7,0.662 7,0.546 2 and 0.570 5,respectively indicating that the overall quality of Eupatorii Herba from Jiangsu,Hebei and Shandong were better.The established QAMS method for simultaneous determination of 11 components in Eupatorii Herba are convenient and accurate.Chemical pattern recognition and GRA method are objective and comprehensive,which can be used to evaluate the quality difference of Eupatorii Herba from different origin.
万方数据
维普
