Anti-inflammatory mechanism of Laggerae Herba based on network pharmacology
This study aims to analyze the components and target pathways of the ethyl acetate fraction of Laggerae Herba(ELH)based on LC-MS and network pharmacology,exploring its anti-inflammatory mechanisms in vitro using an lipopolysac-charides(LPS)-induced RAW 264.7 inflammatory model.The anti-inflammatory activities of various extracts of Laggerae Herba were assessed using the Griess assay.Additionally,LC-MS was utilized to analyze the chemical components of ELH.Network pharmacology aided in predicting and screening the core components,target genes,and principal pathways that medi-ate anti-inflammatory effects,which were then confirmed through molecular docking and Western blot analysis.Results from the Griess assay demonstrated that ELH significantly inhibited NO release in LPS-induced RAW 264.7 cells,achieving an in-hibition rate of 103.07％and identifying it as the active fraction.LC-MS analysis revealed 23 chemical components in ELH,including 21 sesquiterpenes,one flavonoid,and one organic acid.Using four different databases,66 common targets were iden-tified.Protein-protein interaction(PPI)analysis pinpointed six core targets,including interleukin-6(IL-6)and tumor necro-sis factor(TNF),each with a degree value exceeding 40.GO and KEGG enrichment analyses indicated that these core targets could modulate anti-inflammatory effects through the PI3K-Akt,IL-17,NF-κB,and TNF signaling pathways.Molecular doc-king confirmed the strong binding affinity of the 23 chemical components to the core targets.Western blot results indicated significant reductions in Akt phosphorylation and NF-κB expression post-ELH treatment(P＜0.05).In conclusion,ELH can inhibit Akt phosphorylation and,to a certain extent,suppress the activation of the NF-κB signaling pathway induced by LPS in RAW 264.7 cells,thereby exerting potent anti-inflammatory effects.
万方数据
维普
