Mechanism of the protective effect of probiotic fermented Astragali Radix on lipopolysaccharide-induced oxidative damage in normal human colon epithelial cell NCM-460
This study aims to explore the protective effects and molecular mechanisms of probiotic fermented Astragali Radix(F-As)and non-fermented Astragali Radix(As)on the oxidative damage in lipopolysaccharide(LPS)-induced normal hu-man colon epithelial cell NCM-460.The working concentrations of LPS,As and F-As were determined by MTT assay.The changes of oxidative stress-related indexes and antioxidant-related genes at transcription level and protein level in LPS group,As+LPS group and F-As+LPS group were detected and compared respectively.Compared with the control group,reactive oxygen species(ROS)and malondialdehyde(MDA)were significantly increased(P＜0.01)after treatment of LPS,while cell viability,glutathione(GSH),superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and antioxidant-related genes were decreased(P＜0.01).Compared with the LPS group,As+LPS group and F-As+LPS group,the content of ROS and MDA were obviously decreased(P＜0.01),whereas the content of GSH,SOD,T-AOC and antioxidant-related genes were increased(P＜0.01),compared with the LPS group.However,compared with As group,F-As showed a more sig-nificant effect on enhancing these bio-indicators as mentioned above(P＜0.05).The results suggest that both As and F-As can remove excessive ROS and MDA in cells by activating the expression of antioxidant genes,thus playing a protective role in LPS-induced oxidative damage of cells,and F-As is superior to As.
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