This study aims to investigate the chemical constituents of ethyl acetate extracts of Euscaphis japonica dry and ma-ture fruits and their inhibition effect on HeG2 cells.The ethyl acetate part of E.japonica fruits was purified by various column chromatography and semi-prepared HPLC.The isolated compounds were identified by NMR technology.The HepG2 cell model was used to explore the anti-tumor effects of these compounds.Seventeen compounds were isolated and identified as oleanolic acid(1),6β-hydroxybetulinic acid(2),momordic acid(3),2-oxopomolic acid(4),pomolic acid(5),ursolic acid(6),23-aldehydepolomic acid(7),1-oxosiaresinolic acid(8),annurcoic acid(9),maslinic acid(10),kaempferol(11),narin-genin(12),apigenin(13),luteolin(14),eriodictyol(15),methyl gallate(16),protocatechuic acid(17).Among them,compounds 2,9,12-15 were first isolated from the genus.Compounds 2,5,6,10,13 had an inhibitory effect on HepG2 cells.Compound 5 inhibited HepG2 cell proliferation and migration,blocked the cell cycle in G1 phase;promoted the accumulation of reactive oxygen species,reduced the mitochondrial membrane potential,and promoted apoptosis.
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