摘要
研究野鸦椿(Euscaphis japonica(Thunb.)Dippel.)干燥成熟果实的乙酸乙酯部位化学成分及对HepG2细胞的抑制作用.采用多种柱色谱技术和半制备HPLC分离技术,对野鸦椿果实的乙酸乙酯部分进行分离纯化,分离所得化合物运用核磁共振技术进行结构鉴定.并采用HepG2细胞模型对这些化合物的抗肿瘤作用进行探究.最终分离得到17个化合物,分别鉴定为齐墩果酸(1)、6β-羟基白桦脂酸(2)、木鳖子酸(3)、2-氧代坡模酸(4)、坡模酸(5)、熊果酸(6)、23-醛坡模酸(7)、1-氧泰国树脂酸(8)、annurcoic acid(9)、马斯里酸(10)、山柰酚(11)、柚皮素(12)、芹菜素(13)、木犀草素(14)、圣草酚(15)、没食子酸甲酯(16)、原儿茶酸(17).其中,化合物2、9、12～15为首次从野鸦椿属中分离得到.化合物2、5、6、10、13对HepG2细胞有抑制作用.化合物5可抑制HepG2细胞增殖和迁移,阻滞细胞周期于G1期;促进细胞内活性氧的积累,降低线粒体膜电位,促进细胞凋亡.
Abstract
This study aims to investigate the chemical constituents of ethyl acetate extracts of Euscaphis japonica dry and ma-ture fruits and their inhibition effect on HeG2 cells.The ethyl acetate part of E.japonica fruits was purified by various column chromatography and semi-prepared HPLC.The isolated compounds were identified by NMR technology.The HepG2 cell model was used to explore the anti-tumor effects of these compounds.Seventeen compounds were isolated and identified as oleanolic acid(1),6β-hydroxybetulinic acid(2),momordic acid(3),2-oxopomolic acid(4),pomolic acid(5),ursolic acid(6),23-aldehydepolomic acid(7),1-oxosiaresinolic acid(8),annurcoic acid(9),maslinic acid(10),kaempferol(11),narin-genin(12),apigenin(13),luteolin(14),eriodictyol(15),methyl gallate(16),protocatechuic acid(17).Among them,compounds 2,9,12-15 were first isolated from the genus.Compounds 2,5,6,10,13 had an inhibitory effect on HepG2 cells.Compound 5 inhibited HepG2 cell proliferation and migration,blocked the cell cycle in G1 phase;promoted the accumulation of reactive oxygen species,reduced the mitochondrial membrane potential,and promoted apoptosis.
基金项目
厅局级项目-重庆市食品药品检验检测研究院(322019047)
"杏林学者"学科人才科研提升计划-青基人才专项(330022010)
维普
万方数据