摘要
为挖掘药用植物老瓜头的活性成分,对老瓜头全草的乙酸乙酯提取部位进行化学成分分离,并考察其抗氧化和酪氨酸酶抑制活性.采用各种色谱手段从中分离得到10个化合物,通过波谱学方法鉴定结构为3-甲氧基4-羟基苯乙酮(1)、3,4-二甲氧基苯乙酮(2)、6,7-二甲氧基香豆素(3)、6-甲氧基-7-羟基香豆素(4)、对羟基苯丙酮(5)、对羟基苯乙酮(6)、10a,12a-dimethyl-4,4a,4b,10b,11,12-hexahydro-3H-naphtho[2,1-f]chromene-2,8-dione(7)、ent-13S-hydroxy-16-atisene-3,14-dione(8)、3-吲哚甲醛(9)、反式肉桂酸(10).其中化合物7～10为首次从老瓜头中分离得到.分别通过DPPH自由基清除法和酪氨酸酶催化左旋多巴氧化速率法考察化合物的抗氧化活性和酪氨酸酶抑制活性.结果表明化合物1、2、4和5对DPPH自由基有一定的清除能力,其IC50分别为37.3、44.3、51.4、48.2 μg/mL.化合物1、2、3、4和6对酪氨酸酶有显著的抑制作用,其IC50分别为15.7、22.3、8.3、7.1、39.8 μg/mL,均强于阳性对照熊果苷.
Abstract
In order to systematically explore the active ingredients of the medicinal plant Cynanchum komarovii Al.Iljinski,compounds were isolated by various chromatographic techniques from the ethyl acetate extraction of C.komarovii whole plant,and their antioxidant and tyrosinase inhibitory activities were also studied.As a result,ten compounds were obtained and their structures were identified by spectroscopy methods as 3-methoxy-4-hydroxyacetophenone(1),3,4-dimethoxyacetophenone(2),6,7-dimethoxycoumarin(3),6-methoxy-7-hydroxycoumarin(4),p-hydroxypropiophenone(5),p-hydroxyacetophenone(6),10a,12a-dimethyl-4,4a,4b,10b,11,12-hexahydro-3H-naphtho[2,1-f]chromene-2,8-dione(7),ent-13S-hydroxy-16-atisene-3,14-dione(8),3-indole formaldehyde(9),trans-cinnamic acid(10).Compounds 7-10 were isolated from C.kom-arovii for the first time.The antioxidant and anti-tyrosinase activities of these compounds were investigated by using the DPPH free radical method and colorimetric method with L-tyrosine as substrate.The results showed that compounds 1,2,4 and 5 have certain scavenging ability on DPPH free radicals with the IC50 of 37.3,44.3,51.4 and 48.2 μg/mL,respectively.Com-pounds 1,2,3,4 and 6 showed significant tyrosinase inhibitory effects with the IC50 of 15.7,22.3,8.3,7.1 and 39.8 μg/mL,respectively,which were stronger than the positive control arbutin.
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