Study on the chemical constituents from ethyl acetate extract of Ipomoea turbinata seeds
The anti-inflammatory constituents from ethyl acetate extract of Ipomoea turbinata seeds were studied on the basis of lipopolysaccharide-induced mouse mononuclear macrophage RAW 264.7 cell model.Fifteen compounds were isolated from active EtOAc extract by silica gel,Sephadex LH-20,MCI,ODS column chromatography and semi-preparative high perform-ance liquid chromatography(HPLC),and their structures were confirmed by modern spectral techniques and physicochemical properties,as ipalbidine(1),ipalbidinium-4'-O-β-D-(6-O-trans-coumaroyl)-glucoside(2),1,8,15,22-tetraazacyclooctaco-sane-2,9,16,23-tetraketone(3),1,8,15,22,29-pentaaza-pentadecane-2,9,16,23,30-pentanone(4),methyl chlorogenate(5),chlorogenic acid(6),3,5-dicaffeoylquinic acid(7),3,4-dicaffeoylquinic acid(8),caffeic acid(9),3,5-dihydroxy-cinnamic acid(10),4-methoxy-cinnamic acid(11),6-O-(E)-p-hydroxycinnamoyl-1-β-ethyl-glucopyranoside(12),E-3-(3,4-dihydroxy benzylidene)-5-(3,4-dihydroxy phenyl)dihydrofuran-2-one(13),yringaresinol-4-O-β-D-glucopyranoside(14)and pinoresinol 4-O-β-D-glucopyranoside(15).Compounds 3-8 and 10-15 were isolated from this species for the first time,in which eight compounds(3-5,10-13 and 15)were isolated from the Convolvulaceae family for the first time.Bioassay results showed that caffeoylquinic acids(5-8)and phenylpropionic acids(9-12)showed relatively good NO inhibitory activi-ties,with the inhibition rate of 29.07％-40.30％at 50 μmol/L.
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