Protective effect of Olea europaea leaves extract against oxidative damage in HaCaT cells and its antibacterial activity
To investigate the protective effect of Olea europaea leaves extract against oxidative damage in HaCaT cells and its antibacterial activity,the myoglobin protective ratio was used to evaluate the extracellular antioxidant capacity of O.europaea leaves extract against different types of free radicals,the oxidative damage and inflammation model of HaCaT cells was estab-lished to evaluate the protective effect of the extract against oxidative damage to the cells,and the inhibitory effect of O.euro-paea leaves extract on the common pathogenic bacteria was further investigated.The results showed that the O.europaea leaves extract possessed antioxidant effects on hydroxyl radicals,hypochlorite and peroxyl radicals,among which the antioxidant effects on hydroxyl and peroxyl radicals were better.At the cellular level,the extract was able to significantly reduce the intra-cellular free radicals of HaCaT,which could effectively improve the oxidative stress status of HaCaT cells.Meanwhile,0.156-0.625 μg/mL of O.europaea leaves extract was effective in reducing IL-6,IL-1β levels in inflammatory cells,and 0.156-0.312 µg/mL of the extract was able to reduce TNF-α levels.In addition,O.europaea leaves extract was able to effectively inhibit Staphylococcus aureus with low sensitivity to Escherichia coli and Pseudomonas aeruginosa.In conclusion,O.europaea leaves extract has a certain protective effect against oxidative damage in HaCaT cells,and has anti-inflammatory and antibac-terial activities.
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