Optimization of the preparation technics of artemisinin-loaded lactic-co-glycolic acid nanoparticles based on Box-Benhnken response surface method combined with a single factor analysis
This study aims to optimize the preparation technics of artemisinin(ART)loaded lactic-co-glycolic acid(PLGA)nanoparticles(PLGA-ART-NPs).The emulsification-solvent evaporation method was performed to prepare the nanoparticles.The influnences of emulisifier types(polyvinyl alcohol,Tween 80,and poloxamer),the concentrations of the emulisifier,wa-ter-oil ratio,and carrier-drug ratio on the particle size,encapsulation efficiency,and drug loading capacity were analyzed by a single factor analysis.The preparation technics of PLGA-ART-NPs were optimized by using drug loading capacity as the index based on a Box-Benhnken response surface method.The results indicated that polyvinyl alcohol had a better emulsification effect.Emulsifier concentration,water-oil phase ratio,and carrier-drug ratio were chosen as the influence factors and the opti-mal formulations were as follows:emulsifier concentration of 0.017 g/mL,water-oil phase ratio of 18∶1(V/V),and carrier-drug ratio of 10∶1(W/W).Drug loading rate for the nanoparticles prepared under the optimal conditions was detected to be(4.43±0.22)％together with zeta potential at-28.28±2.17 mV.The formulation process optimized in this work,there-fore,is stable,reproducible and feasible,and the prepared nanoparticles possess a satisfying solution stability.
万方数据
维普
