Differential analysis of volatile metabolites in different parts of Cinnamomum camphor by widely-targeted volatilomics method
Cinnamomum camphor is a valuable aromatic plant resource.Studying the volatile components of different parts of C.camphor can provide a basis for further extraction and screening of natural active substances.In this study,fresh leaves and fruits of C.camphor were used as materials,and the headspace solid phase microextraction-gas chromatography-mass spec-trometry(HS-SPME-GC-MS)was used for broad targeted metabolomics analysis.A total of 1 158 secondary metabolites were detected in leaves and fruits,including 16 categories,such as terpenes,esters,heterocyclic compounds,hydrocarbons,ketones and aldehydes,among which terpenes,esters and heterocyclic compounds accounted for the highest proportion of secondary metabolites.PCA and cluster analysis showed that compared with the fruits,there were 141 up-regulated metabolites and 417 down-regulated metabolites.KEGG enrichment analysis showed that the differential metabolites of leaves and fruits mainly in-volved in the biosynthesis of sesquiterpene and triterpenes,monoterpenes,phenylpropanoids and other pathways.The terpenes were the main volatile components in C.camphor while the relative contents of terpenes in leaves and in fruits accounted for 92.33%and 82.73%,respectively.The results revealed the categories and relative content of secondary metabolites in leaves and fruits of C.camphor were quite different.This study provided a theoretical guidance for the fine exploitation and utilization of leaves and fruits of C.camphor.