摘要
研究金果榄的二萜类成分及其抗炎活性.采用多种色谱技术对金果榄乙醇提取物进行分离纯化,通过质谱、核磁、圆二色谱及单晶衍射等方法对化合物结构进行鉴定,并以Griess法测定化合物对脂多糖诱导的小鼠小胶质BV2细胞产生一氧化氮(NO)的抑制活性.从金果榄乙醇提取物乙酸乙酯萃取部分分离得到了七个克罗烷型二萜类化合物,分别为 tinocapill A(1)、tinocapill B(2)、crispene A(3)、floridolide B(4)、古伦宾(5)、tinosporol B(6)、borape-tol A(7).其中化合物1和2为新化合物,活性结果表明,化合物2和6能够显著抑制LPS诱导小鼠BV2细胞释放NO(IC50值分别为9.6和5.8 μmol/L),具有潜在的抗炎活性.
Abstract
This study aims to investigate the diterpenoids of Tinosporae Radix and their anti-inflammatory activity.Various chromatographic techniques were used for isolation of the ethanol extract of Tinosporae Radix.The structures of the com-pounds were identified using MS,NMR,CD,and single X-ray crystal diffraction.The Griess method was used to determine the inhibitory activity of the compounds on the production of nitric oxide(NO)in lipopolysaccharide-induced BV2 cells.Seven clerodane diterpenoids were isolated from the EtOAc part of the ethanol extract of Tinosporae Radix,including tinocapill A(1),tinocapill B(2),crispene A(3),floridolide B(4),columbin(5),tinosporol B(6)and borapetol A(7).Compounds 1 and 2 were new compounds,while the activity results indicated that compounds 2 and 6 could significantly inhibit LPS-in-duced NO release in murine BV2 cells(with IC50 values of 9.6 and 5.8 µmol/L,respectively),showing potential anti-in-flammatory activity.
维普
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