Diterpenoids from Tinosporae Radix and their anti-inflammatory activity
This study aims to investigate the diterpenoids of Tinosporae Radix and their anti-inflammatory activity.Various chromatographic techniques were used for isolation of the ethanol extract of Tinosporae Radix.The structures of the com-pounds were identified using MS,NMR,CD,and single X-ray crystal diffraction.The Griess method was used to determine the inhibitory activity of the compounds on the production of nitric oxide(NO)in lipopolysaccharide-induced BV2 cells.Seven clerodane diterpenoids were isolated from the EtOAc part of the ethanol extract of Tinosporae Radix,including tinocapill A(1),tinocapill B(2),crispene A(3),floridolide B(4),columbin(5),tinosporol B(6)and borapetol A(7).Compounds 1 and 2 were new compounds,while the activity results indicated that compounds 2 and 6 could significantly inhibit LPS-in-duced NO release in murine BV2 cells(with IC50 values of 9.6 and 5.8 µmol/L,respectively),showing potential anti-in-flammatory activity.
Tinosporae Radixextraction and isolationditerpenoidclerodaneanti-inflammatory
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