Chemical constituents from n-butyl alcohol extracts of Moutan Cortex
This study aims to investigate the chemical constitutes of n-butyl alcohol extracts from the 85％ethanol extract of the Moutan Cortex.Compounds were isolated and purified via combinative chromatographic techniques,including macroporous resin,silica gel,Sephadex LH-20 gel,along with mid-pressure and semi-preparative reversed-phase chromatography.The structures of monomeric compounds were elucidated by the data of NMR and MS.Sixteen compounds were obtained and iden-tified as 5-hydroxy-3S-hydroxymethyl-6-methyl-2,3-dihydrobenzofuran(1),(S)-(6-methyl-2,3-dihydrobenzofuran-3-yl)methanol(2),4-thujone-7-hydroxyl-8-O-β-D-glucopyranoside(3),resacetophenone(4),acetoisovanillone(5),2,5-di-hydrox-4-methoxyacetophenone(6),3-(4-hydroxy-3-methoxy phenyl)propan-1,2-diol(7),erythro-1-(3-methoxy-4-hydrox-y-phenyl)-propan-1,2-diol(8),thero-1-(3-methoxy-4-hydroxy-phenyl)-propan-1,2-diol(9),(+)-catechin(10),(+)-catechin-7-O-β-D-glucopyranoside(11),p-hydroxybenzoic acid β-D-glucopyranoside(12),β-D-glucopyranosyl benzoate(13),ethyl gallate(14),gallicin(15)and vanillic acid(16),respectively.Among them,compounds 2,7-9 and 11-16 were obtained from the genus Paeonia for the first time.Activity screening showed that 1 inhibited the NO,TNF-α and IL-6 inflam-matory cytokines levels with IC50 of 15.67±1.20,18.59±1.27 and 15.79±1.20 μmol/L in lipopolysaccharide-induced RAW 264.7 macrophages.
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