Protective effect of polyacetylene compounds from the stem and leaves of Chrysanthemum morifolium Ramat on the HUVECs damage induced by ox-LDL
The aim of this research was to study the protective effect of polyacetylene compounds isolated from the stems and leaves of Chrysanthemum morifolium Ramat on human umbilical vein endothelial cells(HUVECs)damaged by oxidized low density lipoprotein(ox-LDL),and explore the preliminary mechanism of effect.HUVECs cells were treated with ox-LDL(200 μg/mL)to establish the endothelial cell injury model,and polyacetylene compounds 1-10(100 μg/mL)were given at the same time.The cell survival rate was measured by CCK-8,and the target compounds with cell protection were screened.The compounds 5 and 9(12.5,25,50,100 μg/mL)were used to treat the cells for 24 h,CCK-8 was used to detect the cell survival rate,and flow cytometry was used to detect the apoptosis rate and reactive oxygen species(ROS)production rate.The contents of inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the cell culture fluid were detected by enzyme-linked immunosorbent assay(ELISA).In-Cell Western was performed to de-tect the relative expression levels of Nuclear transcription factor-KB(NF-κB)p100/p52 and NF-κBp65.The results showed:compared with the normal control group(Con),the cell survival rate in model group(ox-LDL)was significantly decreased,and the cell apoptosis rate,ROS production rate,inflammatory factors IL-1β,IL-6 and TNF-α were significantly increased(P＜0.01).Compared with ox-LDL group,compounds 5 and 9 significantly increased the survival rate of HUVECs cells,re-duced apoptosis rate,ROS production rate,and inflammatory factors IL-1β,IL-6 and TNF-α(P＜0.05 orP＜0.01),down-regulated the expression of NF-κB2 p100/p52 and NF-κB p65 proteins(P＜0.05 or P＜0.01).In conclusion,polyacetylene glycosides in stems and leaves of C.morifolium can improve ox-LDL induced HUVECs cell damage and dysfunction,and the mechanism of action may be related to NF-κB signaling pathway.
the stems and leaves of Chrysanthemum morifolium Ramatpolyacetylene compoundsHUVECsapoptosis and in-flammationNF-κB
万方数据
维普
