Determination of flavonoids in Paeonia lactifllora flower extracts and their in vitro antioxidant and hypolipidemic activities
This paper presents a comparative study of flavonoid content,in vitro antioxidant activity and lipid-lowering activity of different solvent extracts of Paeonia lactiflora flower.In this paper,the flavonoid content was determined by NaNO2-Al(NO3)3 colorimetric method,the antioxidant capacity of P.lactiflora flower extract was evaluated by DPPH radical scaven-ging rate,ABTS radical scavenging rate,and total reducing capacity,and the lipid-lowering activity of P.lactiflora flower ex-tract was evaluated by pancreatic lipase inhibition capacity,bile-acid-binding capacity,and cholesterol-adsorption capacity.The results showed that the total flavonoid content of the low-eutectic solvent extract was higher than that of the ethanol ex-tract,and also the low-eutectic solvent extraction method improved the antioxidant activity and lipid-lowering activity of P.lac-tiflora flower extracts,and the IC50 values of the low-eutectic solvent extracts for the scavenging of ABTS free radicals were 0.256 mg/mL,and for the scavenging of DPPH free radicals,the IC50 values of the low-eutectic solvent extracts for the scav-enging of DPPH free radicals were 0.296 mg/mL;and the IC50 values for the inhibition of pancreatic Lipase half inhibitory concentration was 0.788 mg/mL,the IC50 values for sodium cholate,sodium taurocholate and sodium glycinate binding were 0.883,0.841,0.850 mg/mL,respectively,and the maximum adsorption of cholesterol was 6.28 mg/g when the dosage of P.lactiflora flower crude extract was 0.5 g.Therefore,low eutectic solvent can be used as a suitable extraction solvent for extrac-ting antioxidant and lipid-lowering components from P.lactiflora flower,and the extracts have better antioxidant and lipid-low-ering potentials,which can provide theoretical basis for the subsequent high-value utilization of P.lactiflora flower.
万方数据
维普
