In order to explore the optimal extraction process of the Rosa rugosa'Hetian'essential oil and its antioxidant and whitening activities,this experiment used rose essential oil as raw material,which was extracted by salt-soaking assisted steam distillation.On the basis of single factor experiment,the effects of salt-soaking time,NaCl mass concentration,and solid-liquid ratio on essential oil extraction were studied,and Box-Behnken response surface method was used to optimize the extraction process parameters.Then,in vitro and cellular level experiments were conducted to verify the antioxidant and whitening activi-ties of rose essential oil obtained from the optimal extraction conditions.The results showed that the extraction amount of es-sential oil was the highest,which could reach 23.35 mg/g(dried flowers)under the conditions of salt soaking time of 2.5 h,NaCl concentration of 5.4％and material-liquid ratio of 1∶7.3(g/mL).Under these conditions,the IC50 value of scavenging DPPH radical of rose essential oil was 7.14 mg/mL.The results of cell experiments showed that the R.rugosa'Hetian'essen-tial oil could reduce the melanin content and inhibit the tyrosinase activity in the α-MSH induced B16F10 cells.To sum up,this study provides an efficient method to extract the R.rugosa'Hetian'essential oil,which proves that rose essential oil has certain antioxidant and whitening activities,further provides a scientific basis for the deep processing,antioxidant and whiten-ing application of the R.rugosa'Hetian'essential oil.
Rosa rugosa'Hetian'essential oilsalt-assisted steam distillationprocess optimizationantioxidationwhitening
维普
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