A new eudesmane-type sesquiterpenoid lactone from branches of Cinnamomum camphora chvar.Borneol
This study aims to study the chemical constituents of Cinnamomum camphora chvar.Borneol branches.Three com-pounds were isolated and purified from the ethyl acetate extraction of C.camphora chvar.Borneol branches by silica gel,Seph-adex LH-20,MCI column chromatography and preparative high-performance liquid chromatography.Their chemical structures were identified as campholactone A(1),northalifoline(2),N-phenylethylbenzamide(3)by spectral techniques and cam-parison with literature data.Compound 1 is a new eucalyptus sesquiterpene lactone with a rare amide substitution at the C-11 position.Compounds 2 and 3 were isolated for the first time from titled plant.The anti-inflammatory activities of compounds 1-3 were evaluated by NO release in LPS-induced RAW 264.7 macrophages and Griess assay,the antitumor activities of com-pounds 1-3 against human liver cancer HepG2 cells were performed by the MTT method.The experimental results showed that compounds 1-3 exhibited varying degrees of inhibitory effects on HepG2 at a concentration of 20 μmol/L,with inhibition rates of 35.78％±0.15％,42.36％±0.58％and 23.95％±0.40％,respectively;compounds 1-3 weakly inhibited the secretion of nitric oxide(NO)with an inhibition rate of less than 20％.
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