The chemical constituents of Streptomyces sp.P325 were investigated.The ethyl acetate extract of its fermentation broth was separated and purified using various chromatography techniques,including silica gel column,ODS reverse gel col-umn,Sephadex LH-20 column,and semipreparative HPLC.Six compounds were isolated,and their structures were identified by spectral data analysis and literature comparison,including two new compounds,tetrahydroxy-octadecanoic acid Ⅰ(1)and tetrahydroxy-hexadecanedioic acid Ⅰ(2),as well as four known compounds:iso-frenolicin B(3),(R)-7-acetyl-3,6-di-hydroxy-8-propyl-3,4-dihydronaphthalen-1(2H)-one(4),methyl p-hydroxybenzoate(5),and isonicotinaldehyde(6).The isolated compounds 1-6 were evaluated for cytotoxic activities.The results indicated that compound 3 exhibited significant in-hibitory activity against HepG2 cells with an IC50 of 13.11 μmol/L.Compound 3 showed a moderate growth inhibitory effect against Hela cells with an IC50 of 25.04 μmol/L.
Streptomyces sp.chemical constituentsseparation and purificationstructural identificationcytotoxic activity
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