Correlation study between accumulation of iridoids and expression of relative genes in Gentiana macrophylla
To research the correlation between the accumulation of iridoids and expression of key enzymes genes of Gentiana macrophylla,UPLC-Q-Exactive MS was used to detect iridoids content,and then quantitative real-time PCR(qRT-PCR)was used to analyze the expression of key enzyme genes in iridoids biosynthesis.Correlation was calculated by SPSS.The results showed that most iridoids were mainly distributed in roots,while loganic acid 11-O-β-glucopyranosyl ester was distributed in leaves and secologanoside was distributed in flowers.The expression of genes was higher in flower parts than in other parts,the expression of loganate O-methyltransferase(LAMT)was the highest in leaves and the expression of genraniol 8-hydroxy-lase(G8O)was the highest in roots.Correlation analysis showed that the relative expression of 7-deoxyloganetic acid glu-cosyltransferase(7-DLGT),isopentenyl-diphosphate delta-isomerase(IDI),secologanin synthase(SLS),mevalonate pyro-phosphate decarboxylase(MVD)and 7-deoxyloganic acid 7-hydroxylase(7-DLH)genes were positively correlated with the contents of iridoids.The expression of the key enzyme genes play an important role in the synthesis of iridoids,which lay a foundation for the regulation of the accumulation of iridoids,and is also an important part of the mechanism of quality forma-tion in the G.macrophylla.
万方数据
维普
