To research the correlation between the accumulation of iridoids and expression of key enzymes genes of Gentiana macrophylla,UPLC-Q-Exactive MS was used to detect iridoids content,and then quantitative real-time PCR(qRT-PCR)was used to analyze the expression of key enzyme genes in iridoids biosynthesis.Correlation was calculated by SPSS.The results showed that most iridoids were mainly distributed in roots,while loganic acid 11-O-β-glucopyranosyl ester was distributed in leaves and secologanoside was distributed in flowers.The expression of genes was higher in flower parts than in other parts,the expression of loganate O-methyltransferase(LAMT)was the highest in leaves and the expression of genraniol 8-hydroxy-lase(G8O)was the highest in roots.Correlation analysis showed that the relative expression of 7-deoxyloganetic acid glu-cosyltransferase(7-DLGT),isopentenyl-diphosphate delta-isomerase(IDI),secologanin synthase(SLS),mevalonate pyro-phosphate decarboxylase(MVD)and 7-deoxyloganic acid 7-hydroxylase(7-DLH)genes were positively correlated with the contents of iridoids.The expression of the key enzyme genes play an important role in the synthesis of iridoids,which lay a foundation for the regulation of the accumulation of iridoids,and is also an important part of the mechanism of quality forma-tion in the G.macrophylla.
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