Establishment of HPLC fingerprint of Epimeredi Indicae Herba and its spectrum-effect relationship of antioxidant and hypoglycemic activities
The HPLC fingerprint of Epimeredi Indicae Herba was established to study its spectrum-effect relationship with an-tioxidant and hypoglycemic activities,so as to provide a basis for the quality evaluation of Epimeredi Indicae Herba.The fin-gerprints of ten batches of Epimeredi Indicae Herba were established by HPLC.The similarity evaluation system for chromato-graphic fingerprint of TCM(2012 edition)was used to evaluate the similarity evaluation.The common peaks were identified by comparison with the reference substance.SPSS 25.0 software was used for cluster analysis and principal component analy-sis.The antioxidant activity was evaluated by DPPH method and ABTS method,and the hypoglycemic activity was evaluated by pNPG method.Grey correlation analysis and bivariate correlation analysis were used to study the spectrum-effect relation-ship.The HPLC fingerprints of ten batches of samples were established,and the similarity was 0.767-1.000.Twenty-one com-mon peaks were identified,and six components were identified.The contents of six components in ten batches of samples were significantly different.Ten batches of samples were clustered into three categories,and six main factors were obtained by prin-cipal component analysis.Ten batches of Epimeredi Indicae Herba had antioxidant and hypoglycemic activities in vitro.The grey correlation degree showed that the correlation degree of twenty-one common peaks with scavenging DPPH,ABTS+free radicals and inhibiting α-glucosidase activity was greater than 0.731.Bivariate correlation analysis showed that peak 3 and peak 17 were significantly positively correlated with DPPH free radical scavenging activity,peak 1 and peak 4 were signifi-cantly positively correlated with ABTS+free radical scavenging activity,peak 1,peak 4 and peak 11(verbascoside)were sig-nificantly positively correlated with α-glucosidase inhibitory activity.This method can effectively analyze the quality of Epim-eredi Indicae Herba from different producing areas,and provide a basis for the screening of antioxidation and hypoglycemic ef-fective material basis and quality control of Epimeredi Indicae Herba.
万方数据
维普
