首页|水稻种子相关细菌的系统发育分析与促生能力评价

水稻种子相关细菌的系统发育分析与促生能力评价

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对15株分离自水稻冀粳14号(90-3)的种子表面及其内生细菌进行了多功能筛选评价和16S rRNA系统发育分析.结果表明,供试菌株都能够溶解无机磷,不能分解有机磷;筛选获得6株具有明显分泌植物生长素吲哚-3-乙酸(IAA)能力的菌株;12株菌株明显产生铁载体,占所测菌株总数的80%,其中7株铁载体产量达到中等水平,通过16S rRNA基因系统发育及聚类分析,供试菌株聚为8个类群,分别属于Rhizobiu、Citrobacter、Xanthomonas、Psedomonas、Bacillus、Pantoea、Microbacterium和Moraxella属.
Analysis on growth- promoting ability and phylogeny with rice seed associated bacteria
In this paper, 15 seed associated strains isolated from rice (Oryza saliva L. Subsp. Japonica 90-3) seeds were studied by means of phylogenetic analysis of 16S rRNA gene and detection the abilities of phosphate solubilization, Indole -3 -Acetic Acid (IAA) secretion and siderophores biosynthesis. The main results were as follows; all the tested strains were able to dissolved inorganic phosphorus, and insoluble organic P; Six strains could secrete IAA obviously; The siderophore production of seven strains reached moderate level. The tested strains were be longing to the genus of Rhizobium, Citrobacter, Xan-thomonas, Psedomonas, Bacillus, Pantoea, Microbacterium and Moraxella according to 16S rRNA gene phylogenetic analysis.

rice seed associated bacteriaphylogenyphosphate solubilizationIAA secretionsiderophores biosynthesis.

曹艳花、徐凤花、陈小忠、张晓霞、韦善君、马晓彤

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东北农业大学资源与环境学院,黑龙江哈尔滨150030

中央民族大学生命与环境科学学院,北京100081

中国农业科学院农业资源与农业区划研究所,中国农业微生物菌种保藏管理中心,北京100081

水稻种子相关细菌 系统发育 溶磷 IAA分泌 铁载体合成

国家自然科学基金中央级公益性科研院所基本科研业务费专项

309000012011-11

2011

中国土壤与肥料
中国农业科学院农业资源与农业区划研究所 中国植物营养与肥料学会

中国土壤与肥料

CSTPCDCSCD北大核心
影响因子:1.197
ISSN:1673-6257
年,卷(期):2011.(5)
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