Effects of long-term different fertilization on soil enzyme activity and soil microbe in yellow paddy fields
A long-term (28 years) fertilizer experiment was carried out in the yellow paddy in Fujian Scientific Observing and Experimental Station of Arable Land Conservation,Ministry of Agriculture,China,to study effects of long-term different fertilization systems on soil enzyme activity and soil microbe.The field experiment included four treatments:(1) unfertilized control (CK) ; (2) chemical fertilizers (NPK) ; (3) chemical fertilizers plus cattle manure (NPKM) ; (4)chemical fertilizer plus straw (NPKS).The results showed that compared with CK,the soil enzyme activities including hydrogen peroxidase,urease,invertase and phosphatase in all fertilization treatments were all increased more or less,and the invertase activities were significantly increased under the NPKM treatment,the urease activities were significantly increased under the NPKS treatment.The amount of soil microflora was also increased more or less in all fertilization treatments,especially the amount of actinomycetes showed a significant increase.Among the fertilization treatments (NPK,NPKM,NPKS),only NPKS led to a significant increase of soil bacterial amount,and only NPKM could significantly increase soil fungal amount.All fertilization treatments had important impacts on fungal community,compared with CK.However,chemical fertilizer alone had no influence on soil bacterial community,but organic amendment led to noticeable change.The dominant community of soil actinomycetes could be affected by the application of cattle manure.These findings indicated combined use of chemical fertilizers with manure or crop straw was better to raise soil enzyme activity and soil microbial amount,leading to enhancement of soil biological fertility.