Screening and identification of a rhizosphere phosphate-solubilizing bacteria and its growth-promoting effect
Phosphorus played an important role in growth and development of plants.In order to obtain high-quality phosphate-solubilized growth-promoting bacteria,the rhizosphere soil of peach garden in Pinggu district,Beijing was used as the raw material,and the strains with high phosphate-solubilizing and growth-promoting capacity were screened out.The strain that could produce phosphate-solubilizing ring was screened out by plate test and then cultured in liquid state.The maximum phosphate-solubilizing ability of the strain was determined by molybdenum-antimony colorimetric method,and the relationship between the phosphate-solubilizing ability of the strain and pH change was explored.At the same time,the growth-promoting characteristics of the strain were studied by pot experiment.Three phosphate-solubilizing bacteria(JP01,JP03 and PG62)were isolated from the rhizosphere soil of peach tree,and preliminarily identified as Aspergillus niger,Pseudomonas aeruginosa and Corynebacterium pallidum.Among them,A.niger JP01 had excellent phosphate solubilizing ability,its ability to dissolve Inorganic phosphorus was 112.52 mg/L,the conversion amount of organic phosphorus was 145.50 mg/L,and the maximum phosphate solubilizing ability was 95.74 mg/L.During the process of culture,the pH of the culture solution corresponding to the phosphate-solubilizing bacteria decreased significantly.The pot experiment showed that A.niger could increase the plant height,stem diameter and underground dry weight of maize seedlings,increase the total phosphorus content of the plants and had a certain effect on soil available phosphorus.The results of this study showed that A.niger met the technical specifications for the development of phosphate-solubilizing microbial fertilizers.
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