Study on the enhancement of soil effective phosphorus content by dual-domain β-propeller alkaline phytase
Excessive use of inorganic phosphate fertilizers in agricultural production has triggered a number of problems such as soil slumping,and searching for alternatively sustainable phosphate fertility sources is necessary.Organic phosphate in soil exists mainly in form of phytate which plants cannot directly absorb and utilize.Releasing the phytate phosphorus using microbial phytase can provide an eco-friendly solution for sustainable phosphate fertility in agriculture.The method of present work was to add dual-domain β-propeller alkaline phytase(phyHT)from Bacillus sp.HJB17 to investigate its soil stability and test its effect on increasing the effective phosphorus content of soil,improving soil physical and chemical properties and promoting plant growth.The results showed that the soil microorganisms had little effect on phyHT,which was stable over a wide range of temperature(20-40℃)and pH(6.0-9.0)for 12 hours and functions as an efficient catalyst in moist soils.After exogenous addition of phyHT:(1)Soil phytate content was decreased by 17.00％and effective phosphorus content was increased by 22.52％;(2)Soil pH was decreased,the conductivity of soil was increased,CaCO3 content was decreased by 3.55 percentage point,and soil organic matter,ammonium nitrogen and available potassium content were increased by 10.54％,18.38％and 27.49％,respectively;(3)Wheat seedling growth length,dry matter accumulation,and total phosphorus content of shoot and root tissues were significantly increased.Therefore,exogenous addition of phyHT can promote soil phytate hydrolysis,increase soil effective phosphorus content and improve soil phosphorus effectiveness,at the same time,it can improve the physical and chemical properties of the soil to a certain extent and promote plant growth.
β-propeller phytasesenzyme activityplant growth-promotingphosphorus effectiveness in soils
万方数据
维普
