Effect on bacterial community of complex oligosaccharide applied in cucumber growth system
Oligosaccharides,a kind of plant stimulating hormone,had the effects of improving crop stress resistance and promoting crop growth.To elucidate the effects of complex oligosaccharides application on increasing yield and efficiency of cucumber plants,the effects of complex oligosaccharides on plant photosynthesis characteristics,yield and the microbial communities in the leaves,roots,and soil around the roots of cucumber at three growth stages were analyzed after complex oligosaccharides preparations applied in the early and growth stages of cucumber.Using a greenhouse plot experiment,three treatment groups were set up:clear water control,chemical conventional treatment and complex oligosaccharide treatment.Cucumber leaves,roots,and soil samples were collected during the flowering,fruiting and seedling stages,respectively.Cucumber yield,chlorophyll content and antioxidant enzyme activity were measured at each sampling period,and high-throughput sequencing was performed to detect the bacterial community structure and composition of the samples.Compared with clear water control,the application of complex oligosaccharides increased the yield of cucumber per plant by 4.96％.The content of chlorophyll in cucumber leaves was significantly increased,and the relative value of chlorophyll content(SPAD)in the complex digosaccharide treatment was significantly increased by 6.14％-11.46％compared to other treatments.At the same time,the activity of superoxide dismutase in the leaves was increased by 39.9％-81.5％,the activity of catalase was increased by 8.4％-40.4％,and the activity of peroxidase was increased by 1.19％-18.5％.The high-throughput sequencing analysis results showed that the application of complex oligosaccharides increased the abundance of Sphingomonas sp.and Methylobacterium sp.in the leaf microbial community,both of which have the effects of promoting plant growth and assisting plant disease resistance.In addition,complex oligosaccharides increased the abundance of biocontrol bacteria Bacillus sp.in the microbial community of the rhizosphere soil.The application of complex oligosaccharides in the early and growth stages of cucumber could increase cucumber yield and plant antioxidant enzyme activity,delay plant aging,and increase the abundance of beneficial bacteria in the plant growth system.It could provide basic data for further revealing the mechanism of action of complex oligosaccharides.
万方数据
维普
