Effects of Bacillus amyloliquefaciens W48 on garlic yield and soil quality improvement
In order to clarify the effect of microbial agents on improving the environment of garlic producing area and improving the yield and quality of garlic,conventional fertilization combined with Bacillus amyloliquefaciens W48 fermentation broth was used to treat the soil in garlic field.The yield and quality related indexes of garlic were measured,and the changes of soil physical and chemical properties and biotransformation related enzymes were monitored.The content of phthalate ester plasticizers(PAEs)in soil and garlic treated with microbial agents was quantitatively analyzed.The results showed that W48 strain has the ability to secrete indoleacetic acid,as well as the potential to dissolve phosphorus and produce iron carriers.The application of W48 microbial agent significantly increased the contents of organic matter,alkali-hydrolyzed nitrogen,available phosphorus and available potassium in the rhizosphere soil,improved the trophic environment in the rhizosphere,increased the activities of soil alkaline phosphatase,sucrase and urease,and improved soil nitrogen and phosphorus use efficiency.Compared with the control,the garlic plots treated with microbial agents increased the yield by 10％,and the content of soluble sugar and vitamin C were improved,among which the soluble sugar increased by 30％.Di-n-butyl phthalate(DBP)and di-2-ethyl hexyl phthalate(DEHP)were the main types of PAEs detected in soil and garlic,with the average concentration of 1874.05 and 337.12 μg/kg,respectively.The residual amount of PAEs in garlic was generally high.The highest DBP was 666.57 μg/kg,followed by diisobutyl phthalate(DIBP)which was 409.93 μg/kg and butyl benzyl phthalate(BBP)which was 433.65 μg/kg.W48 exhibited significant PAEs degradation ability in vitro,and the bacterial agent treatment could significantly reduce the concentration of PAEs in soil,but had no significant effect on the content of PAEs in garlic.The removal rates of DBP,DEHP and dimethyl phthalate(DMP)were 25.5％,76.1％and 48.4％,respectively.The results of this study can provide technical support for remediation and improvement of soil pollutants in garlic producing area.
garlicmicrobial agentphthalate ester plasticizerssoil improvementincrease production
万方数据
维普
